Project description:Here, in a time-course phosphoproteome analysis on Arabidopsis seedlings exposed to control or high ambient temperature, we observed reduced levels of diverse proteins over time, which could be in part due to transcription, translation and/or degradation. In addition, we observed differential phosphorylation of the LRR F-box protein SLOMO MOTION (SLOMO) at two serine residues. We demonstrated that SLOMO is a negative regulator of hypocotyl growth, and protein-protein interaction studies revealed possible interactors of SLOMO.
Project description:The hypocotyl of Arabidopsis seedlings shows rhythmic periods of elongation. The patterns of elongation are controlled by a combination of internal factors, such as the circadian clock, and external factors such as light. In a previous study we had found that two transcription factors, PIF4 and PIF5 are important integrators of clock and light signals for the control of elongation. Here we use microarrays to find genes that are correlated with elongation and that are controlled by PIF4 and/or PIF5.
