Next Generation Sequencing of Chimeric antigen receptor T (CAR-T) cells polarized under Th9 condition

ABSTRACT: Isolated human T cells was stimulated by anti CD3/CD28 Dynabeads and polarized under Th1 (IL2) or Th9 (IL4 plus TGF beta) condition.After 24 hours, cells were transduced with lentivirus. Medium were renewed every 2 days and cells were maintained at a concentration of 1 million per milliliter. On day 16 of culture total RNA was isolated from CAR-T cells using RNeasy Mini kits (Qiagen) following the manufacturer's instruction. Quality of total RNA was evaluated using RNA 6000 Nano LabChip. Qualified RNA was subject to next generation sequencing.

ORGANISM(S): Homo sapiens

PROVIDER: GSE156075 | GEO | 2020/09/18

REPOSITORIES: GEO