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Examination of NK, ILC, macrophage and dendritic cells from human adipose tissue in lean and obese donors.

ABSTRACT: We sorted NK, ILC, macrophage and dendritic cells from human adipose tissue and did single cell RNA sequencing on these cells. We observed cell type compositon changes and expression changes in the lean and obese donors.

ORGANISM(S): Homo sapiens

PROVIDER: GSE156110 | GEO | 2021/03/09

REPOSITORIES: GEO

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Examination of human adipose celltypes in lean and obese donors

Project description:We did single cell RNA sequencing on three lean and three obese donors. For each donor, we sorted CD45 positive and CD45 negative cells and prepared 10X scRNA-seq libaries. We observed cell type compositon changes and expression changes in the lean and obese donors.

2021-03-09 | GSE155960 | GEO

Examination of NK, ILC, macrophage and dendritic cells from human adipose tissue in lean and obese donors.

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Examination of human adipose celltypes in lean and obese donors

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Transcription profiling of human cumulus cells in vitro isolated from polycystic ovary syndrome lean and obese individuals reveals PCOS in lean and obese individuals is associated with different molecular pathways

Project description:Objective: The etiology of PCOS is mostly unknown. Existing data support both genetic and environmental factors in its pathogenesis. Design: Prospective case - control study. Setting: University Hospital. Patients: 25 patients undergoing IVF-ICSI treatment. Intervention: Genome-wide oligonucleotide microarray technology was used to study differential gene-expression patterns of cultured human cumulus cells from IVF patients divided into 4 groups according to disease state (PCOS vs. Control) and BMI (Obese vs. Lean). Results: Two differential PCOS gene expression profiles were established: Lean-Type was formed by comparing PCOS lean (PL) vs. non-PCOS lean (NL) individuals; Obese-Type was formed by comparing PCOS obese (PO) vs. non-PCOS (NO) obese patients. Conclusions: Different molecular pathways are associated with PCOS in Lean and Obese individuals, as demonstrated by gene expression profiling of cumulus cells. Our findings provide insights into the molecular pathogenesis of PCOS. We used microarrays to study the gene expression of human cultured cumulus cells. We compared the genes expression of lean PCOS, Obese PCOS, lean controls and obese controls. Different molecular pathways are associated with PCOS in Lean and Obese patients. Experiment Overall Design: Cumulus cells obtained from woman undergoing IVF/ICSI. Following oocyte retrieval, cumulus cells were stripped from the oocyte, in preparation for the ICSI process, with a micropipette. After 48h in culture the cumulus cells were collected for RNA extraction and hybridization on Affymetrix microarrays. We compered the expression profile of 4 groups - lean PCOS, obese PCOS, lean controls and obese controls.

2008-06-11 | E-GEOD-10946 | biostudies-arrayexpress

Adipose tissue stromal cells from lean, obese, and formerly obese mice

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Whole blood expression profiling of obese diet-sensitive, obese diet-resistant and lean human subjects

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CD4+ T cells mRNA profiles of lean and obese mice

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Mouse-adapted SARS-CoV-2 infected lungs from lean or obese ob/ob mice

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