Transcriptomics,Genomics

Dataset Information

23

L. acidophilus NCFM™, B. lactis 420, L. salivarius Ls-33 and E. coli O157:H7 effect on intestinal epithelial cells


ABSTRACT: Analysis of differentiated Caco-2 intestinal epithelial cell line cocultured with probiotics L. acidophilus NCFM™, B. lactis 420, L. salivarius Ls-33 bacterial cells or treated with cell-free supernatant, and with E. coli O157:H7 cell-free supernatant. Lactobacillus and Bifidobacterium are important genera suggested to be beneficial for human health and E. coli O157:H7 is a pathogen causing hemorrhagic colitis and hemolytic uremic syndrome. Results provide insight into the mechanisms underlying the beneficial effects of probiotics on intestinal epithelial cells and a comparison to pathogenic E. coli. Overall design: Differentiated Caco-2 cells were cocultured with either L. acidophilus NCFM™, B. lactis 420, and L. salivarius Ls-33 bacteria for 8 hours or with L. acidophilus NCFM™, B. lactis 420, L. salivarius Ls-33, and E. coli O157:H7 cell-free media for 24 hours. As a control a medium-only treatment was used. For probiotics seven samples were analyzed in duplicate with intestinal epithelial cells: three treated with cell-free supernatant, three co-cultures and a control. For EHEC, six samples were analyzed similarly with intestinal epithelial cells: three supernatant samples and three control samples all done as single replica.

INSTRUMENT(S): [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array

SUBMITTER: Ilana Saarikko 

PROVIDER: GSE15636 | GEO | 2009-04-11

SECONDARY ACCESSION(S): PRJNA115635

REPOSITORIES: GEO

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Publications

Analysis of the human intestinal epithelial cell transcriptional response to Lactobacillus acidophilus, Lactobacillus salivarius, Bifidobacterium lactis and Escherichia coli.

Putaala H H   Barrangou R R   Leyer G J GJ   Ouwehand A C AC   Hansen E Bech EB   Romero D A DA   Rautonen N N  

Beneficial microbes 20100901 3


The complex microbial population residing in the human gastrointestinal tract consists of commensal, potential pathogenic and beneficial species, which are probably perceived differently by the host and consequently could be expected to trigger specific transcriptional responses. Here, we provide a comparative analysis of the global in vitro transcriptional response of human intestinal epithelial cells to Lactobacillus acidophilus NCFM™, Lactobacillus salivarius Ls-33, Bifidobacterium animalis s  ...[more]

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