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Elevation of JAML Promotes Diabetic Kidney Disease by Modulating Podocyte Lipid Metabolism

ABSTRACT: Podocytes, highly differentiated glomerular epithelial cells, are essential for the maintenance of glomerular filtration barrier. Lipid accumulation in podocytes is a major determinant of proteinuric kidney disease. By RNA-sequencing analysis, we observed the significant changes of genes important in regulating cellular lipid homeostasis in podocytes with HG treatment. We generated two mouse podocyte cell lines stably transduced with either a sh-NC lentiviral construct or a shRNA lentiviral construct designed to target JAML RNA to investigate the consequences on the gene expression profile of JAML knockdown in mouse podocytes.

ORGANISM(S): Mus musculus

PROVIDER: GSE156367 | GEO | 2020/08/18

REPOSITORIES: GEO

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