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Epigenetic profiling to identify a human effector regulatory T cell core signature [H3K27ac]

ABSTRACT: We investigated the active enhancer landscape of human Treg in an autoimmune-associated inflammatory environment, i.e. synovial fluid (SF) from inflamed joints of Juvenile Idiopathic Arthritis (JIA) to improve our understanding of human effector Treg programming.

ORGANISM(S): Homo sapiens

PROVIDER: GSE156417 | GEO | 2021/02/23

REPOSITORIES: GEO

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Transcriptome profiling to identify a human effector regulatory T cell core signature

Project description:We investigated the transcriptomic landscape of human Treg in an autoimmune-associated inflammatory environment, i.e. synovial fluid (SF) from inflamed joints of Juvenile Idiopathic Arthritis (JIA) to improve our understanding of human effector Treg programming.

2021-02-23 | GSE161426 | GEO

Epigenetic profiling to identify a human effector regulatory T cell core signature [H3K4me1]

Project description:We investigated the gene expression profile and active enhancer landscape of human Treg in an autoimmune-associated inflammatory environment, i.e. synovial fluid (SF) from inflamed joints of Juvenile Idiopathic Arthritis (JIA) to improve our understanding of human effector Treg programming.

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Proteome of neutrophils upon migration towards inflamed synovial fluid

Project description:Analysis of neutrophil proteomic alterations induced by migration towards inflamed joints in juvenile idiopathic arthritis (JIA). In this experiment neutrophil proteomes were investigated after migration towards JIA synovial fluid in an in vitro model of a synovial membrane, compared to neutrophils incubated in synovial fluid without migration. The migration model consisted of transwell inserts with human knee synoviocytes on the undersides and HMEC endothelial cells on the insides, placed in wells containing medium with 10 % JIA synovial fluid.

2022-02-15 | PXD029129 | Pride

Single-cell transcriptomes and T cell receptor sequences of memory T lymphocytes from the peripheral blood and the synovial fluid of patients with juvenile idiopathic arthritis.

Project description:Antigen-experienced (or memory) T lymphocytes were isolated from the synovial fluid of inflamed joints and the peripheral blood of patients with juvenile idiopathic arthritis (JIA). After flow-sort of CD4+CD45RO+CD25- (Tcon), CD4+CD45RO+CD127loCD25+ (Treg) and CD8+CD45RO+ T cells, single-cell RNA-sequencing was conducted to perform paired analyses of gene transcription and T cell receptor (TCR) repertoires in the same cells. The study aimed to identify T memory cells that were stimulated by antigens in inflamed joints of JIA patients and which perpetuate chronic inflammation by expression pro-inflammatory genes. In addition, the study aimed at understanding the clonal relationships of T cells differentiating at chronically inflamed sites, as well as to identify blood-circulating T cell clones that shared gene expression and antigen-specificity with T cells in inflamed joints in order to improve patient stratification and diagnostics of patients with chronic inflammatory diseases in the future.

2020-11-27 | GSE160097 | GEO

Proteome characterization of synovial fluid polarized healthy monocytes

Project description:This project involves proteome characterization of healthy monocytes polarized (activated) with synovial fluid (SF) versus serum from patients with juvenile idiopathic arthritis (JIA). The objective is to investigate the influence of SF on monocytes at the proteome level, using serum polarized monocytes as a control. Monocytes were isolated from peripheral blood mononuclear cells of healthy donors following negative selection and were subsequently polarized overnight with 20% of a pool of either serum or SF. They were subsequently detached and used for proteome analysis.

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Conventional DC1 (cDC1) are Strongly Enriched, Quiescent and Relatively Tolerogenic in Local Inflammatory Arthritis

Project description:We sequenced (bulk RNA-seq) conventional (c)DC1, cDC2 and monocytes from a local inflammatory site, namely synovial fluid (SF) from patients suffering from a chronic inflammatory condition, Juvenile Idiopathic Arthritis (JIA). To be able to compare to steady state, we also sequenced cDC2 and monocyte subsets from peripheral blood of healthy controls.

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Transcriptomic Analysis of Isolated Monocytes from heathy donors and JIA patinets using Nanostring Technology

Project description:Among immune cells, activated monocytes play a detrimental role in chronic and viral-induced inflammatory pathologies. The uncontrolled activation of monocytes and the subsequent excessive production of inflammatory factors damage bone-cartilage joints in Juvenile Idiopathic Arthritis (JIA), a childhood rheumatoid arthritis (RA) disease. The moderate beneficial effect of current therapies and clinical trials highlights the need of alternative strategies targeting monocytes to treat RA disease. Here, we show that targeting CXCR4 with small amino compound such as the histamine analog clobenpropit (CB) inhibits spontaneous and induced-production of a set of key inflammatory cytokines by monocytes isolated from blood and synovial fluids of JIA patients. Moreover, daily intraperitoneal CB treatment of arthritic mice results in significant decrease in circulating inflammatory cytokine levels, immune cell infiltrates, joints erosion, and bone resorption leading to reduction of disease progression. These overall data show that targeting CXCR4 with CB-like molecules may represent a promising therapeutic option for chronic and viral-induced inflammatory diseases.

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2009-03-04 | GSE15083 | GEO

Childhood Onset Rheumatic Disease Gene Expression Profile

Project description:Total RNA from peripheral blood mononuclear cells (PBMC) and neutrophils from children with juvenile dermatomyositis (JDM) and juvenile idiopathic arthritis (JIA) were separately compared to pediatric control samples. Keywords: pediatric rheumatic disease, blood, PBMC, neutrophil, JIA, JDM

2009-03-25 | GSE11083 | GEO

Response of synovial fibroblasts to TNFα and IL6/sIL6R

Project description:Synovial fibroblasts (SF) are the main mesenchymal cell type constitutive in human synovial tissue. SF contribute to the homeostasis of normal joints by synthesizing extracellular matrix components and secreting the specific components of synovial fluid. SF are essential players in RA pathophysiology, they are the primary source of IL6 in the RA synovium contributing to perpetuate the inflammation in the joint. We used microarrays analysis to characterize the effector pro-inflammatory response of SF to TNFα and IL6/sIL6R signaling.

2021-06-01 | GSE132830 | GEO

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