High-Content Imaging-Based Pooled CRISPR Screens in Mammalian Cells

ABSTRACT: We developed a microscopy-based approach, which we name optical enrichment, to select cells displaying a particular CRISPR-induced phenotype by automated imaging-based computation, mark them by photo-activation of an expressed photo-activatable fluorescent protein, and then isolate the fluorescent cells using fluorescence-activated cell sorting (FACS). One proof-of-principle screen, a nuclear size screen, a FSC screen and a H2B-mGFP screen were performed to validate the method.

ORGANISM(S): Homo sapiens

PROVIDER: GSE156623 | GEO | 2021/01/12

REPOSITORIES: GEO