Project description:We identified global DNA binding properties of EWS-FLI1 in mouse Ewing sarcoma. GGAA microsatellites were found as binding sites of EWS-FLI1 but with less frequency than that in human Ewing sarcoma, and genomic distribution is not conserved between human and mouse.
Project description:This study aims to characterize the H3K27me3 modification in wild type and Gls CD19cre B KO or Efnb1 ai3 B KO or Kdm6b OE CD83+ly75+GCB cells, and find the potential mechanism of the Gls CD19cre B KO or Efnb1 ai3 B KO or Kdm6b OE CD83+ly75+GCB cells。
Project description:EWSR1-FLI1 is a chimeric transcription factor resulting from the pathognomonic translocation present in Ewing sarcoma cells. Here, we silenced EWSR1-FLI1 in different Ewing sarcoma cell lines. RNA from SKNMC, TC71 and MHH-ES1 cells was extracted 96h post transfection (siCT or siEWSR1-FLI1) or prior doxycycline (day 0) and 7 days after inducing silencing of EWSR1-FLI1 with doxycycline in ASP14 cells. RNA-seq was performed for all conditions.
Project description:To examined the role of FLI1 in glioblastoma cells and identify FLI1-associated target molecules, FLI1 knockdown cells were used for RNA-seq.
