Genomics

Dataset Information

22

Chromosomal duplication in the grandifolia-D mutants of Arabidopsis thaliana, tiling array data


ABSTRACT: The number of cells in an organ is a major factor for the determination of organ size. However, genetic basis of cell number determination is not well understood. Three grandifolia-D (gra-D) mutants of Arabidopsis thaliana developed huge leaves containing two- to three-fold increased number of cells of the wild type. Tiling array and microarray analysis of gra-D mutants suggested that genes found in a lower part of chromosome 4 were upregulated, suggesting the occurrence of segmental chromosomal duplications in the gra-D mutants. These region contain positive regulators of cell proliferation such as AINTEGUMENTA (ANT) and cyclin genes such as CYCD3;1. Overall design: genomic DNA was extracted using DNeasy plant mini kit (Qiagen) from vegetative part of seedlings grown in vitro. DNA from wild type (wt, control) and the 3 grandifolia mutants (gra1-D, gra2-D and gra3-D) were submitted to tiling array hybridization.

INSTRUMENT(S): [At35b_MR] Affymetrix Arabidopsis Tiling 1.0R Array

SUBMITTER: Nathalie Gonzalez  

PROVIDER: GSE15725 | GEO | 2009-06-02

SECONDARY ACCESSION(S): PRJNA122999

REPOSITORIES: GEO

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Publications

Impact of segmental chromosomal duplications on leaf size in the grandifolia-D mutants of Arabidopsis thaliana.

Horiguchi Gorou G   Gonzalez Nathalie N   Beemster Gerrit T S GT   Inzé Dirk D   Tsukaya Hirokazu H  

The Plant journal : for cell and molecular biology 20090605 1


The number of cells in an organ is a major factor that specifies its size. However, the genetic basis of cell number determination is not well understood. To obtain insight into this genetic basis, three grandifolia-D (gra-D) mutants of Arabidopsis thaliana were characterized that developed huge leaves with two to three times more cells than the wild-type. Genetic and microarray analyses showed that a large segmental duplication had occurred in all the gra-D mutants, consisting of the lower part  ...[more]

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