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Genome-wide Kdm1a binding in X chromosome by high throughput sequencing [ChIP-Seq]

ABSTRACT: We analyzed KDM1A (LSD1) occupancy in the Xi during somatic cell reprogramming of female mouse cells. We use MEFs from hybrid embryos by crossing male Mus spretus and female Mus musculus domesticus C57BL/6J to distiguish genome DNA from the Xi. We found a possible physical and/or functional regulation of KDM1A during the X chromosome reactivation in the intiation site on the Xi.

ORGANISM(S): Mus musculus

PROVIDER: GSE157479 | GEO | 2022/01/01

REPOSITORIES: GEO

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Genome-wide transcripts from X chromosome by high throughput sequencing [RNA-Seq]

Project description:We analyzed a transcriptional landscape of the Xi during somatic cell reprogramming of female mouse cells. We use MEFs from hybrid embryos by crossing male Mus spretus and female Mus musculus domesticus C57BL/6J to monitor allele-specific transcripts from the Xi. We could capture the earliest phase of X chromosome reactivation and found that a subset of genes clustered near the centromere display an early reactivation on the Xi.

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