Genome-scale deconvolution of RNA structure ensembles

ABSTRACT: RNA structure heterogeneity is a major challenge when querying RNA structures with chemical probing. We introduce DRACO, an algorithm for the deconvolution of coexisting RNA conformations from mutational profiling experiments. Analysis of the SARS-CoV-2 genome using dimethyl sulfate mutational profiling with sequencing (DMS-MaPseq) and DRACO, identifies multiple regions that fold into two mutually exclusive conformations, including a conserved structural switch in the 3′ untranslated region. This work may open the way to dissecting the heterogeneity of the RNA structurome.

ORGANISM(S): Severe acute respiratory syndrome coronavirus 2

PROVIDER: GSE158052 | GEO | 2020/12/23

REPOSITORIES: GEO