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CRISPR screening of porcine sgRNA library identifies host factors associated with Japanese encephalitis virus replication

ABSTRACT: We design a porcine genome-scale CRISPR/Cas9 knockout (PigGeCKO) library containing 85,674 single guide RNAs targeting 17,743 protein-coding genes, 11,053 long ncRNAs, and 551 microRNAs. Subsequently, we use the PigGeCKO library to identify key host factors facilitating JEV infection in porcine cells.

ORGANISM(S): Sus scrofa

PROVIDER: GSE158247 | GEO | 2020/09/21

REPOSITORIES: GEO

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