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MeRIP-seq of mRNAs in MEFs with or without nutrient starvation

ABSTRACT: To investigate the dynamic m6A modification during starvation-induced autophagy, we performed MeRIP-seq in MEFs with or without nutrient deprivation and identified the variations of m6A modification on mRNAs. Briefly, the total polyadenylated mRNAs in MEFs cultured with or without nutrient-deprived medium for 2 hours were isolated using RNAiso plus reagent (Takara) followed Dynabeads mRNA Purification Kit (Invitrogen). The m6A RNA immunoprecipitation was performed using GenSeq m6A RNA IP Kit. NEBNext Ultra II Directional RNA Library Prep Kit (NEB) was used for library generation. Each experiment was conducted in two biological replicates. Methylated sites on peaks were identified by MACS2 peak-calling software (Zhang et al., 2008). Differentially methylated sites were identified by diffReps (Shen et al., 2013) with default setting.

ORGANISM(S): Mus musculus

PROVIDER: GSE158268 | GEO | 2021/12/30

REPOSITORIES: GEO

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Transcriptome-wide N6-methyladenosine modification profiling in patients with recurrent implantation failure (RIF)

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