Single-cell characterization of transcriptomic heterogeneity in lymphoblastoid cell lines

ABSTRACT: We report single-cell RNA sequencing datasets for three lymphoblastoid cell lines (LCLs) prepared by infection of primary B cells with Epstein-Barr Virus (EBV). Inter- and intra-sample transcriptional variance is observed with respect to immunoglobulin heavy chain class; host gene sets involved in survival, proliferation and differentiation; and viral replication state. We propose that the observed heterogeneity arises from the dynamic nature of host-pathogen interactions, donor-specific compositions of primary B cells, and other experimental factors such as stochastic clonal evolution in cell culture conditions.

ORGANISM(S): human gammaherpesvirus 4 Homo sapiens

PROVIDER: GSE158275 | GEO | 2021/02/15

REPOSITORIES: GEO