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Metabolism-based resistance to herbicides in black-grass

ABSTRACT: Resistance to herbicides in weeds can be due to alteration(s) in the gene encoding the herbicide target site, or to herbicide degradation via a deviation in plant general metabolism. If target-site-based resistance is easy to study, the multigenic control of metabolism-based resistance renders it much more complex to study. Metabolism-based resistance to herbicides represents the major part of herbicide resistance in black-grass. Its most likely basis is an overexpression of genes encoding enzymes degrading herbicides. We thus seek to identify such overexpressed genes by comparing the transcriptomes of resistant and sensitive black-grass individuals belonging to an F2 line segregating for two resistance genes. Given there are no genomic tools developed for black-grass, this approach will use heterologous hybridisation onto a wheat Affymetrix microarray. Comparison using heterologous hybridisation onto a wheat whole-genome microarray of transcriptome of three pools of black-grass plants obtained 2h30 after herbicide spraying at field rate. The three pools correspond to: · Sensitive plants (killed by herbicide). · Moderately resistant plants (growth impaired by herbicide but plants still alive) · Resistant plants (growth unimpaired by herbicide)

ORGANISM(S): Triticum aestivum Alopecurus myosuroides

PROVIDER: GSE15864 | GEO | 2010/05/04

SECONDARY ACCESSION(S): PRJNA116967

REPOSITORIES: GEO

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Metabolism-based resistance to herbicides in black-grass

Project description:Resistance to herbicides in weeds can be due to alteration(s) in the gene encoding the herbicide target site, or to herbicide degradation via a deviation in plant general metabolism. If target-site-based resistance is easy to study, the multigenic control of metabolism-based resistance renders it much more complex to study. Metabolism-based resistance to herbicides represents the major part of herbicide resistance in black-grass. Its most likely basis is an overexpression of genes encoding enzymes degrading herbicides. We thus seek to identify such overexpressed genes by comparing the transcriptomes of resistant and sensitive black-grass individuals belonging to an F2 line segregating for two resistance genes. Given there are no genomic tools developed for black-grass, this approach will use heterologous hybridisation onto a wheat Affymetrix microarray. Comparison using heterologous hybridisation onto a wheat whole-genome microarray of transcriptome of three pools of black-grass plants obtained 2h30 after herbicide spraying at field rate. The three pools correspond to: Â· Sensitive plants (killed by herbicide). Â· Moderately resistant plants (growth impaired by herbicide but plants still alive) Â· Resistant plants (growth unimpaired by herbicide) 6 arrays - wheat

2010-05-27 | E-GEOD-15864 | biostudies-arrayexpress

A key role for a glutathione transferase in multiple herbicide resistance in grass weeds

2013-03-13 | GSE42065 | GEO

A key role for a glutathione transferase in multiple herbicide resistance in grass weeds

Project description:The gene encoding a protein (AmGSTF1) associated with multiple herbicide resistance (MHR) in black-grass was transgenically expressed in Arabidopsis thaliana.The goal of this study was to determine if AmGSTF1 could elicit an MHR phenotype in the transgenic host. Affymetix microarrays were used to detect changes in transcriptional regulation between amgstf1-expressors and wild-type Arabidopsis plants (ecotype Col0). Total RNA was isolated from amgstf1-expressors and wild-type Arabidopsis plants (ecotype Col0) respectively, grown under identical growth room conditions. Performed in biological triplicate.

2013-03-13 | E-GEOD-42065 | biostudies-arrayexpress

Transcriptome analysis indicates the involvement of herbicide-responsive and plant–pathogen interaction pathways in the development of resistance to ACCase inhibitors in Apera spica-venti

Project description:Background: The continuous use of the herbicides contributes to the emergence of the resistant populations of numerous weed species that are tolerant to multiple herbicides with different modes of action (multiple resistance) which is provided by non-target-site resistance mechanisms. In this study, we addressed the question of rapid acquisition of herbicide resistance to pinoxaden (acetyl CoA carboxylase inhibitor) in Apera spica-venti, which endangers winter cereal crops and has high adaptation capabilities to inhabit many rural locations. To this end, de novo transcriptome of Apera spica-venti was assembled and RNA-sequencing analysis of plants resistant and susceptible to pinoxaden treated with this herbicide was performed. Results: The obtained data showed that the prime candidate genes responsible for herbicide resistance were those encoding 3-ketoacyl-CoA synthase 12-like, UDP-glycosyltransferases (UGT) including UGT75K6, UGT75E2, UGT83A1-like, and glutathione S-transferases (GSTs) such as GSTU1 and GSTU6. Also, such highly accelerated herbicide resistance emergence may result from the enhanced constitutive expression of a wide range of genes involved in detoxification already before herbicide treatment and may also influence response to biotic stresses, which was assumed by the detection of expression changes in genes encoding defence-related proteins, including receptor kinase-like Xa21. Moreover, alterations in the expression of genes associated with methylation in non-treated herbicide-resistant populations were identified. Conclusion: The obtained results indicated genes that may be involved in herbicide resistance. Moreover, they provide valuable insight into the possible effect of resistance on the weed interaction with the other stresses by indicating pathways associated with both abiotic and biotic stresses.

2023-04-22 | GSE204857 | GEO

Transcriptome analysis indicates the involvement of herbicide-responsive and plant-pathogen interaction pathways in the development of resistance to ACCase inhibitors in Apera spica-venti

2023-04-22 | GSE204788 | GEO

Alopecurus myosuroides

Project description:Metabolism-based resistance to herbicides in black-grass

| PRJNA116967 | ENA

Transcription profiling of field grown wheat treated with 3 herbicides

Project description:Wheat plants, grown in the field, were treated with 3 herbicides commonly applied on this crop to determine potential changes in gene expression.

2005-09-01 | E-MEXP-330 | biostudies-arrayexpress

Metabolomic Aspects of Conservative and Resistance-related Elements of Response to Fusarium culmorum in the Grass Family

Project description:BACKGROUND: Fusarium head blight (FHB) is a serious fungal disease of crop plants due to substantial yield reduction and production of mycotoxins in the infected grains. The breeding progress in increasing resistance with maintaining a high yield is not possible without a thorough examination of the molecular basis of plant immunity responses.METHODS: LC-MS based metabolomics approaches powered by three-way ANOVA and differentially accumulated metabolites (DAMs) selection, correlation network and functional enrichment were conducted on grains of resistant and susceptible to FHB genotypes of barley and wheat as well as model grass Brachypodium distachyon (Bd) still poorly known at metabolomic level.RESULTS: We selected common and genotype-specific DAMs in response to F. culmorum inoculation. Immunological reaction at metabolomic level was strongly diversified between resistant and susceptible genotypes. DAMs common for all tested species from porphyrins, flavonoids and phenylpropanoids metabolic pathways were highly correlated and reflects conservativeness in FHB response in Poaceae family. Resistant related DAMs belonged to different structural classes including tryptophan derived metabolites, pirimidines, amino acids proline and serine as well as phenylpropanoids and flavonoids. Physiological response to F. culmorum of Bd was close to barley and wheat genotypes however, metabolomic changes were strongly diversified. CONCLUSIONS: Combined targeted and untargeted metabolomics provides comprehensive knowledge about significant elements of plant immunity with potential of being molecular biomarkers of enhance resistance to FHB in grass family. Thorough examination of Bd21 metabolome in juxtaposition with barley and wheat diversified genotypes facilitate their setting as model grass for plant-microbe interaction.

2022-10-27 | MTBLS1748 | MetaboLights

Transcription profiling of wheat treated with 3 herbicides grown in controlled conditions

Project description:Wheat plants grown in controlled conditions were treated with 3 herbicides commonly applied on this crop to determine the potential effects of these compounds on gene expression.

2005-09-01 | E-MEXP-329 | biostudies-arrayexpress

Transcriptome response to safener treatment in rice cell cultures

Project description:Safeners are an important group of agrochemicals that can extend the selective use of existing herbicides into a wider range of cereals by protecting the crops from chemical injury. The recently released aromatic sulphonamide safeners cyprosulfamide and metcamifen were tested for their ability to protect rice plants from the phytotoxicity caused by clodinafop-propargyl, a herbicide normally only used in wheat. Transcriptome studies in rice cultures demonstrated that whereas cyprosulfamide had a negligible effect on gene expression over a 4 h exposure, metcamifen perturbed the abundance of 590 transcripts. Analysis of the inducible genes suggested that safening elicited similar gene families to those associated with specific biotic and abiotic stresses, notably those elicited by abscisic acid, salicylic acid and methyl-jasmonate.

2019-06-01 | GSE124666 | GEO

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