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Telomere behavior in a hybrid yeast

ABSTRACT: Telomeres and the protein/RNA complexes involved in maintaining them are rapidly evolving systems across eukaryotes. Using two Saccharomyces species, S. cerevisiae and S. bayanus, we provide evidence that the telomere systems of these two closely related yeasts have evolved significantly apart and that the gene in one species can not maintain the set-point of telomere length of the other species in the hybrid.

ORGANISM(S): Saccharomyces bayanus Saccharomyces bayanus x Saccharomyces cerevisiae Saccharomyces cerevisiae

PROVIDER: GSE15877 | GEO | 2009/05/01

SECONDARY ACCESSION(S): PRJNA117033

REPOSITORIES: GEO

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Telomere behavior in a hybrid yeast

Project description:Telomeres and the protein/RNA complexes involved in maintaining them are rapidly evolving systems across eukaryotes. Using two Saccharomyces species, S. cerevisiae and S. bayanus, we provide evidence that the telomere systems of these two closely related yeasts have evolved significantly apart and that the gene in one species can not maintain the set-point of telomere length of the other species in the hybrid. Each array has co-hybridized RNA vs DNA for the indicated species (BB9=S. bayanus, CC5=S. cerevisiae) or hybrid (BC11). The cultures for RNA preparation were grown to midlog phase at the indicated temperature.

2010-05-19 | E-GEOD-15877 | biostudies-arrayexpress

Telomere behavior in a hybrid yeast

Project description:Telomere behavior in a hybrid yeast

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Project description:Telomeres are the nucleoprotein complexes located at the tips of eukaryotic chromosomes, composed of repetitive DNA (TTAGGG in vertebrates), and coated by a set of proteins collectively known as the shelterin complex. Dysfunctional telomeres resemble DSBs and they have been observed during ageing and cancer and a number of pathological conditions. Apart from telomeric repeat-containing RNA (TERRA), a non-coding UUAGGG-rich transcript starting from promoters located in the subtelomeric region, in mammals no other transcripts at telomeres have been characterized so far. Here we show that in mammals telomere dysfunction (analysed using TRF2+/- and TRF2-/- mouse embryonic fibroblasts) induces the transcription of telomeric DDRNAs (tDDRNAs) from both DNA strands. To characterize the length and sequence of tDDRNAs, we devised and employed an innovative method for target enrichment of RNA, based on in solution capture of low abundance small RNA species followed by next generation sequencing, developed in our laboratories. By this approach, we observed that telomere deprotection induced the accumulation of small RNA species generated from the transcription of both telomere strands and including the expected DDRNA size range products. Importantly, 20-23 nucleotide RNAs displayed a base bias at both 5’ and 3’ ends significantly different from the telomeric locus suggesting a regulated processing.

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Project description:Four hybrid yeast strains isolated from a variety of industrial substrates were hybridized to an array-CGH platform containing probes to query the whole genomes of seven different Saccharomyces species. For most of the strains we found evidence of multiple interspecific hybridization events and multiple introgressed regions. The strains queried were GSY205 (isolated from a cider fermentation), GSY505 (a contaminant from a lager beer fermentation), GSY2232 (a commercial wine yeast strain), and GSY312 (a commercial lager beer strain). Additionally, 3 different rare viable spores derived from laboratory-created interspecific S. cerevisiae-S. bayanus (aka S. uvarum) hybrids were queried, before and after evolution in chemostats, via S. cerevisiae-S. bayanus microarrays.

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Project description:We examined differential expression of genes within 10MBs of telomeres in myoblasts with long or short telomeres We offer telomere looping with telomere length as a partial mechanistic explanation for the changes gene expression that is observed. Compare expression of genes within 10MB of the telomere in normal myoblasts with long (15 kb) and short (6 kb) telomeres.

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Project description:RNAi, a gene-silencing pathway triggered by double-stranded RNA, is conserved in diverse eukaryotic species but has been lost in the model budding yeast, Saccharomyces cerevisiae. We report that RNAi is present in other budding-yeast species, including Saccharomyces castellii and Candida albicans. These species use noncanonical Dicer proteins to generate siRNAs, which mostly correspond to transposable elements and YM-BM-4 subtelomeric repeats. In S. castellii, RNAi mutants are viable but have excess YM-BM-4 mRNA levels. In S. cerevisiae, introducing Dicer and Argonaute of S. castellii restores RNAi, and the reconstituted pathway silences endogenous retrotransposons. These results identify a novel class of Dicer proteins, bring the tool of RNAi to the study of budding yeasts, and bring the tools of budding yeast to the study of RNAi. Employ high-throughput sequencing of endogenous small RNAs from the budding yeasts Saccharomyces castellii, Kluyveromyces polysporus, Candida albicans, Saccharomyces cerevisiae, and Saccharomyces bayanus.

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2022-03-04 | PXD032093 | iProX

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