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Host-informed molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine [Ribo-seq]

ABSTRACT: We analyzed total RNA and ribosome footprints collected from a K. phaffii strain secreting the VP8-P[6] serotype of the rotavirus antigen and compared against a strain secreting the VP8-P[4] serotype for reference.

ORGANISM(S): Komagataella phaffii

PROVIDER: GSE159336 | GEO | 2021/05/05

REPOSITORIES: GEO

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Host-informed molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine [RNA-seq]

Project description:We analyzed the transcriptomes of K. phaffii strains secreting the VP8-P[6] serotype of the rotavirus antigen, and compared against null strains as well as strains secreting the VP8-P[4] or VP8-P[8] serotype in order to diagnose bottlenecks limiting robust secretion of P[6].

2021-05-05 | GSE159325 | GEO

Host-informed molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine

Project description:This SuperSeries is composed of the SubSeries listed below.

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Scalable, methanol-free manufacturing of the SARS-CoV-2 receptor binding domain in engineered Komagataella phaffii

Project description:Prevention of COVID-19 on a global scale will require the continued development of high-volume, low-cost platforms for the manufacturing of vaccines to supply on-going demand. Vaccine candidates based on recombinant protein subunits remain important because they can be manufactured at low costs in existing large-scale production facilities that use microbial hosts like Komagataella phaffii (Pichia pastoris). Here, we report an improved and scalable manufacturing approach for the SARS-CoV-2 spike protein receptor binding domain (RBD); this protein is a key antigen for several reported vaccine candidates. We genetically engineered a manufacturing strain of K. phaffii to obviate the requirement for methanol-induction of the recombinant gene. Methanol-free production improved the secreted titer of the RBD protein by >5x by alleviating protein folding stress. Removal of methanol from the production process enabled scale up to a 1,200 L pre-existing production facility. This engineered strain is now used to produce an RBD-based vaccine antigen that is currently in clinical trials and could be used to produce other variants of RBD as needed for future vaccines.

2021-12-15 | GSE183408 | GEO

Host-informed molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine [Ribo-seq]

Project description:Host-informed molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine [Ribo-seq]

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Translation complex profiling for bacteria [Ribo-seq]

Project description:Ribosomes were arrested during initiation, elongation and termination complex formation using formaldehyde crosslinking. The complex protected footprints were isolated after MNase treatment and sequenced.

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Streptococcus agalactiae LC-MSMS

Project description:The human-derived serotype Ⅴ ST1 GBS strains NNA038 and NNA048 was isolated from a amniotic fluid of full-term pregnant woman who suffered from premature rupture of membrane in China.Serotype Ia ST7 GBS strain YM001 is an attenuated strain ,its parent strain HN016 was isolated from an outbreak epidemical disease in tilapia from China.HN016_KO_D2 is a knockout strain from Serotype Ia ST7 GBS strain HN016.

2019-11-12 | PXD011206 | Pride

Host-informed molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine

Project description:Host-informed molecular engineering improves antigen quality and enables integrated manufacturing of a trivalent subunit vaccine

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GAS M6 serotype, wild-type vs covR mutant

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A study on common carp gene expression response to cooling stress using Ribo-Seq

Project description:Common carp (~0.5kg) previously held for over 3 months at 25°C were randomly placed into two large aquarium tanks, one which remained throughout at 25°C (control) and the other was cooled over 3 days to 11°C for 23 days. Replicate specimens from both tanks were then killed at up to 17 timepoints after cooling and kidney tissue was sampled, and ribosomal footprints were quantified as a function of time after cooling using Ribo-Seq.

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Listeria monocytogenes strain F2365 has reduced ability to survive stress while maintaining virulence characteristics

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