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S. pyogenes wild type vs. CvfA- mutant

ABSTRACT: The overall gene expression of a CvfA- mutant of Streptococcus pyogenes grown in C medium was compared to that of the wild type (HSC5spc). Stationary phase cells (8 hrs post-inoculation) were used because the gene expression of the CvfA- mutant was most different from that of the wild type at stationary phase, while the difference of gene expression between the CvfA- mutant and the wild type was negligible at exponential phase.

ORGANISM(S): Streptococcus pyogenes

PROVIDER: GSE15938 | GEO | 2009/05/08

SECONDARY ACCESSION(S): PRJNA116985

REPOSITORIES: GEO

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S. pyogenes wild type vs. CvfA- mutant

Project description:The overall gene expression of a CvfA- mutant of Streptococcus pyogenes grown in C medium was compared to that of the wild type (HSC5spc). Stationary phase cells (8 hrs post-inoculation) were used because the gene expression of the CvfA- mutant was most different from that of the wild type at stationary phase, while the difference of gene expression between the CvfA- mutant and the wild type was negligible at exponential phase. Two biological replicates (two different RNA sets extracted from the CvfA- mutant and the wild type at different day were used): 399773,399887,399888 vs. 399889,399890,399891 Technical replicates (Microarrays were hybridized with the same RNA with the same dye): 399773 and 399887; 399889 and 399890 Dye swap (Microarrays were hybridized with the same RNA but Cy3 and Cy5 dyes were swapped): 399773, 399887 vs. 399888; 399889, 399890 vs. 399891

2010-06-25 | E-GEOD-15938 | biostudies-arrayexpress

Rgg-dependent transcriptional regulation in SF370 Streptococcus pyogenes strain

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Counteractive balancing of transcriptome expression involving CodY and CovRS in Streptococcus pyogenes

Project description:Streptococcus pyogenes (group A Streptococcus, GAS) responds to environmental changes in a manner that results in an adaptive regulation of the transcriptome. Global transcriptional regulators are able to integrate important extracellular and intracellular information and are responsible for modulation of the transcriptional network. The roles of several global transcriptional regulators in adaptation and virulence gene expression have been described. In this study we used microarray to investigate the regulatory roles of CodY and CovRS played in Streptococcus pyogenes. keywords: genetic modification Streptococcus pyogenes NZ131 wild-type cells, ΔcodY, ΔcovRS and ΔcodYcovRS strains were grown in C-medium until mid-exponential phase or early-stationary phase. The transcriptional profile of the whole genome was examined with microarray.

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