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Genome-wide analysis of chromatin accessibility in human tonsillar B cell subsets

ABSTRACT: We performed ATAC-Seq on three freshly dissociated human tonsils sorted to purity into the following B-cell subsets: CD19+ naïve (IgD+/CD38-), memory (IgD-/CD38-), germinal center light zone (IgD-/CD38mid/CD83+/CXCR4lo), germinal center dark zone (IgD-/CD38mid/CD83lo/CXCR4hi), and plasma cells (IgD-/CD38hi). We used these data to derive differentially accessible sites between B-cell subsets and relative to EBV-immortalized B cell lines (LCLs). These studies revealed similarities between LCLs and both DZ and LZ GC cells at the BCL2A1 (BFL1) locus that formed the impetus for further three-dimensional chromatin studies, which are the focus of the associated manuscript.

ORGANISM(S): Homo sapiens

PROVIDER: GSE159673 | GEO | 2020/10/21

REPOSITORIES: GEO

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