Transcriptomics,Genomics

Dataset Information

41

Changes in gene expression of hMSCs and NOD/scid mouse lung after IV infusion of hMSCs


ABSTRACT: Quantitative assays for human DNA and mRNA were used to examine the paradox that intravenously (IV) infused human multipotent stromal cells (hMSCs) can enhance tissue repair without significant engraftment. After 2 X 106 hMSCs were IV infused into mice, most of the cells were trapped as emboli in lung. The cells in lung disappeared with a half-life of about 24 hr but < 1,000 cells appeared in 6 other tissues. The hMSCs in lung up-regulated expression of multiple genes with a large increase in the anti-inflammatory protein TSG-6. After myocardial infarction, IV hMSCs but not hMSCs transduced with TSG-6 siRNA decreased inflammatory responses, reduced infarct size, and improved cardiac function. IV administration of recombinant TSG-6 also reduced inflammatory responses and reduced infarct size. The results suggest improvements in animal models and patients after IV infusions of MSCs are at least in part explained by activation of MSCs to secrete TSG-6. Overall design: 3 samples containing either mouse lung and human cells or only mouse lung run on a 3 human and 3 mouse arrays. 1) control NOD/scid mouse lung 2) in vitro mix of NOD/scid mouse lung and hMSCs 3) NOD/scid mouse lung 10h after IV infusion of 2 million hMSCs

REANALYSED by: GSE119128

INSTRUMENT(S): [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array

SUBMITTER: Joni Ylostalo  

PROVIDER: GSE15969 | GEO | 2009-05-07

SECONDARY ACCESSION(S): PRJNA115547

REPOSITORIES: GEO

altmetric image

Publications

Intravenous hMSCs improve myocardial infarction in mice because cells embolized in lung are activated to secrete the anti-inflammatory protein TSG-6.

Lee Ryang Hwa RH   Pulin Andrey A AA   Seo Min Jeong MJ   Kota Daniel J DJ   Ylostalo Joni J   Larson Benjamin L BL   Semprun-Prieto Laura L   Delafontaine Patrice P   Prockop Darwin J DJ  

Cell stem cell 20090701 1


Quantitative assays for human DNA and mRNA were used to examine the paradox that intravenously (i.v.) infused human multipotent stromal cells (hMSCs) can enhance tissue repair without significant engraftment. After 2 x 10(6) hMSCs were i.v. infused into mice, most of the cells were trapped as emboli in lung. The cells in lung disappeared with a half-life of about 24 hr, but <1000 cells appeared in six other tissues. The hMSCs in lung upregulated expression of multiple genes, with a large increas  ...[more]

Similar Datasets

| PRJNA115547 | ENA
2011-01-13 | E-GEOD-26567 | ArrayExpress
2011-01-13 | GSE26567 | GEO
2009-06-30 | GSE13073 | GEO
2014-05-05 | E-GEOD-57266 | ArrayExpress
2009-07-04 | E-GEOD-13073 | ArrayExpress
2012-12-05 | E-GEOD-42687 | ArrayExpress
2014-06-23 | E-GEOD-52563 | ArrayExpress
| GSE52563 | GEO
2006-07-07 | GSE4953 | GEO