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Interleukin enhancer-binding factor 2 promotes cell proliferation and DNA damage response in metastatic melanoma

ABSTRACT: This study aimed to understand the role of ILF2 upregulation in metastatic melanoma cutaneous progression and DNA damage response. The goal of RPPA analysis was to determine the protein expression profiles in DP-0574 and IM-0223 melanoma cell lines with ILF2 overexpression (ILF2-OV) or control empty vector (EV). By analysis of RPPA in both metastatic melanoma cell lines, we found that ILF2-OV controls significantly increased RAD50 expression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE159692 | GEO | 2022/04/15

REPOSITORIES: GEO

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MiR-200a Regulates CDK4/6 Inhibitor Effect by Targeting CDK6 in Metastatic Melanoma

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protein levels in BRAF inhibitor resistance melanoma cells treated with PF3758309

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2017-07-31 | GSE96753 | GEO

protein levels in BRAF/MEK inhibitor resistance melanoma cells treated with PF3758309 [CR]

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An oncolytic virus–delivered TGFβ inhibitor overcomes the immunosuppressive tumor microenvironment

Project description:Oncolytic viruses (OV) are promising forms of immunotherapy that have demonstrated clinical benefit in difficult-to-treat cancers such as metastatic melanoma. However, their adoption in other malignancies has been limited, in part, due to poorly understood mechansims of therapeutic resistance. Here, bulk RNA-seq was performed on oncolytic vaccinia virus-sensitive and -resistant murine head and neck squamous cell carcinomas (MEERvvS and MEERvvR, respectively) to explore potential means of OV resistance. These results corroborated a potential role for TGF-beta mediated stabilization of immunosuppressive regulatory T cells in the tumor microenvironment of OV-resistant MEERvvR-bearing mice. Subsequently, treatment with oncolytic vaccinia virus engineered to expess a dominant negative TGF-β signaling inhibitor (VVtgfbi) restored sensitivity to OV-mediated cell death among MEERvvR tumors. Single-cell RNA seq performed on CD45+ immune cells isolated from tumors suggests TGF-β inhibition may also reduce the presence and activity of myeloid-derived suppressor cell populations within the tumor microenvironment.

2023-07-27 | GSE188506 | GEO

Investigation of ST8SIA1 expression in icaritin-treated and untreated melanoma cell lines

Project description:We identified that upregulation of ST8SIA1 promotes melanoma brain metastasis. The goal of this RNA-seq is to analyze the ST8SIA1 expression in icaritin-treated and untreated melanoma cell lines (DP-0574 and WP-0614 cell lines) In vitro functional studies demonstrate that ST8SIA1 overexpression promotes cell proliferation and colony formation and that this can be halted by icaritin treatment.

2024-04-30 | GSE130956 | GEO

Targeted inhibition of human breast cancer cell lines selected as model systems of ERBB2-positive/EGFR high breast cancer

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2018-01-02 | PXD007265 | Pride

Knockdown of NLGN4X in melanoma cells

Project description:NLGN4X is a postsynaptic cell adhesion molecule that is important for synaptic function and has recently been implicated to play a role in various neuronal disorders. We detected high NLGN4X expression in non-metastatic melanoma cell lines and low NLGN4X expression in metastatic melanoma cells by Western blot and real time PCR analysis. Here, we used whole genome mRNA profiling to investigate the consequences of NLGN4X loss for melanoma progression. Therfore, we determined gene expression changes after NLGN4X knockdown and identified HIF1 pathway up-regulation in two non-metastatic melanoma cell lines.

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