RNA binding of Saccharomyces cerevisiae Ssd1

ABSTRACT: We quantified the exact RNA binding sites of the Ssd1 protein in Saccharomyces cerevisiae, in exponential growth and heat shock conditions, using the CRAC protocol. We used a His-TEV-protein A tag (HTP) on the C-terminal of the genomic copy of Ssd1, with the 3'UTR replaced by the 3'UTR/terminator from the K. lactis Ssd1 homolog, followed by a KlURA3 selection marker.

ORGANISM(S): Saccharomyces cerevisiae BY4741

PROVIDER: GSE159835 | GEO | 2020/10/23

REPOSITORIES: GEO