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RNAseq in immortalized human podocyte cell line following PRDM15 CRISPR knock-out

ABSTRACT: We recently identified variants in PRDM15 in affected individuals with proteinuric kidney disease. To determine the downstream pathways mediated by PRDM15 knock-down, PRDM15 knock-out immortalized podocyte cell lines were generated using CRISPR/Cas genome editing. Two different guide RNAs were utilized to generate two different cell lines, while a scramble guide RNA was utilized as control. We performed RNAseq and identified 151 differentially regulated genes, which coalesced upon pathways involved in renal development.

ORGANISM(S): Homo sapiens

PROVIDER: GSE159978 | GEO | 2021/02/01

REPOSITORIES: GEO

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