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A facile dCAS9-M. EcoGII system for targeted DNA 6mA methylation in living cell

ABSTRACT: DNA methylation plays vital roles in multiple biological processes. N6-methyladenine (6mA), a prevalent DNA modification in prokaryotes, has recently been shown to play functional roles in eukaryotic cells with diverse distributions. However, the prevalence of 6mA and its functional roles at specific genomic regions have yet to be clearly established. Here, we developed a facile and versatile approach for locus-specific 6mA installation. We fused a nuclease dead Cas9 (dCas9) with an engineered N6‑deoxyadenine methyltransferase M. EcoGII, which could achieve rapid and efficient 6mA methylation at targeted genomic locus. By using our system, we have successfully introduced 6mA onto tested gene promoter regions. We have observed a repressive role of 6mA in gene transcription regulation. We also validated a positive correlation between 6mA and H3K9Me3 in tumor suppressor genes such as RASSF2 and AKAP6. This new approach provides a direct way for depiction of basic roles of 6mA in specific genomic locus and expands the toolkit for epigenetic regulation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE160065 | GEO | 2025/12/01

REPOSITORIES: GEO

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A facile dCAS9-M. EcoGII system for targeted DNA 6mA methylation in living cell

Project description:A facile dCAS9-M. EcoGII system for targeted DNA 6mA methylation in living cell

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