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Identifying candidate genes whose expression is regulated by CbrAB and NtrBC in Pseudomonas fluorescens SBW25

ABSTRACT: Histidine is a good source of nutrient for many bacteria, but its utilization poses a significant challenge as it delivers excess nitrogen over carbon. The rate of histidine catabolism (hut) must thus be tightly regulated. Here, in Pseudomonas fluorescens SBW25, we show that the CbrAB two-component system directly activates the transcription of hut genes, while mediating succinate-induced carbon catabolite repression of hut at the translational level via the CbrAB-CrcYZ-Hfq/Crc regulatory cascade. When growing in minimal salt medium supplemented with histidine and succinate (the most preferred carbon source), the CbrAB-mediated promoter activity is weak; and the global nitrogen regulator NtrBC plays the dominant role in directly activating hut transcription.

ORGANISM(S): Pseudomonas fluorescens

PROVIDER: GSE160152 | GEO | 2020/12/12

REPOSITORIES: GEO

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Identifying candidate genes whose expression is regulated by the HutC transcriptional repressor in Pseudomonas fluorescens SBW25

Project description:HutC in Pseudomonas is a representative member of the HutC/GntR family of transcriptional regulators, which possess a N-terminal winged helix-turn-helix (wHTH) DNA-binding domain and a C-terminal substrate binding domain. HutC is generally known to represses expression of histidine utilization (hut) genes through binding to the PhutU promoter with urocanate (the first intermediate of the histidine degradation pathway) as the direct inducer. Here we first describe the detailed molecular interactions between HutC and its PhutU target site in a plant growth promoting bacterium P. fluorescens SBW25, and further show that HutC possesses specific DNA-binding activities with many targets in SBW25 genome. Subsequent RNA-seq analysis and phenotypic assays revealed an unexpected global regulatory role of HutC for successful bacterial colonization in planta.

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Identifying candidate genes whose expression is regulated by CbrAB and NtrBC in Pseudomonas fluorescens SBW25

Project description:Identifying candidate genes whose expression is regulated by CbrAB and NtrBC in Pseudomonas fluorescens SBW25

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Novel targets of the CbrAB/Crc carbon catabolite control system revealed by transcript abundance in Pseudomonas aeruginosa [BSM]

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2012-03-31 | E-GEOD-33245 | biostudies-arrayexpress

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