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CPEB4-mediated translational regulation of LPS response in Bone Marrow Derived Macrophages (BMDMs)

ABSTRACT: Here we show the transcriptomic profile of the LPS response in BMDMs wild type and KO for the RNA-binding protein CPEB4.

ORGANISM(S): Mus musculus

PROVIDER: GSE160246 | GEO | 2022/05/05

REPOSITORIES: GEO

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CPEB4-mediated translational regulation of LPS response in BMDMs

Project description:Here we show that the RNA-binding protein CPEB4 sustains the expression of anti-inflammatory factors in LPS-stimulated BMDMs, by binding to the corresponding mRNAs and promoting their stabilization. in macrophages.

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CPEB4-mediated translational regulation of LPS response in Bone Marrow Derived Macrophages (BMDMs)

Project description:CPEB4-mediated translational regulation of LPS response in Bone Marrow Derived Macrophages (BMDMs)

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CPEB4-mediated translational regulation of LPS response in BMDMs

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Project description:Purpose: The purpose of this study was to compare the BMDMs transcriptome in the control LPS and LPS plus BHB group in order to identify the critical pathway that contributed to the inhibiton of BHB on M1 polarization. Methods: 1× 106 BMDMs were pre-treated with PBS or BHB for 1 hours before LPS administration. Four hours later, BMDMs were harvested for RNA preparation. Results: Among 16650 mapped genes, 7325 differentially expressed genes were identified, including 244 LPS inducible BHB down regulated named class A and 537 LPS repressed BHB up regulated genes named class B. The genes in class A were mainly TLR4 and TNF signaling pathway, while genes in class B were associated with negative regulation of transcription and NF-KB signaling. The GSEA showed that BHB have the similar profiles chnage on BMDMs with TSA. Conclusions: Our study revealed that BHB down regulated TLR4 and NF-KB pathway related genes to inhibited M1 polarization, and BHB have a similar effect on BMDMs with TSA through comparing the genetic changes of two substance caused in cells.

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Gene expression from bone-marrow derived macrophages.

Project description:Gene expression from WT and NFAT5 KO primary macrophage cultures. Keywords: Bone-marrow derived macrophages. We analyzed 4 arrays from each condition: unstimulated WT BMDMs, LPS stimulated WT BMDMs, unstimulated KO BMDMs, LPS stimulated KO BMDMs.

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