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Secretory Cells Dominate Airway CFTR Expression and Function in Human Airway Superficial Epithelia

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE160675 | GEO | 2020/12/16

REPOSITORIES: GEO

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Secretory Cells Dominate Airway CFTR Expression and Function in Human Airway Superficial Epithelia [bulk RNA]

Project description:Purpose: To identify the cell types that contribute to CFTR expression and function within the proximal-distal axis of the normal human lung. Methods: Single cell RNA-seq (scRNA-seq) was performed on freshly isolated human large and small airway epithelial cells. Single cell-based RNA in situ hybridization (scRNA-ISH) and quantitative RT-PCR (scqRT-PCR) were performed for validation. In vitro culture systems correlated CFTR function to cell types. Lentiviruses were used for cell-type specific transduction of wild-type CFTR in CF cells. Results: scRNA-seq identified secretory cells as dominating CFTR expression in normal human large and particularly small airways, followed by basal cells. Ionocytes expressed the highest CFTR levels but were rare, while expression in ciliated cells was infrequent and low. scRNA-ISH, and scqRT-PCR confirmed the scRNA-seq findings. CF lungs exhibited distributions of CFTR and ionocytes similar to normal controls. CFTR-mediated Cl- secretion tracked secretory cell, not ionocyte, densities. Further, the nucleotide-purinergic regulatory system that controls CFTR-mediated hydration was associated with secretory cells. Lentiviral transduction of wild-type CFTR produced CFTR-mediated Cl- secretion in CF airway secretory cells but not ciliated cells. Conclusions: Secretory cells dominate CFTR expression and function in human airway superficial epithelia. CFTR therapies may need to restore CFTR function to multiple cell types, with a focus on secretory cells.

2020-12-16 | GSE160674 | GEO

Secretory Cells Dominate Airway CFTR Expression and Function in Human Airway Superficial Epithelia [single-cell RNA-seq]

2020-12-16 | GSE160673 | GEO

Secretory Cells Dominate Airway CFTR Expression and Function in Human Airway Superficial Epithelia

Project description:Secretory Cells Dominate Airway CFTR Expression and Function in Human Airway Superficial Epithelia

| PRJNA673903 | ENA

Secretory Cells Dominate Airway CFTR Expression and Function in Human Airway Superficial Epithelia [bulk RNA]

Project description:Secretory Cells Dominate Airway CFTR Expression and Function in Human Airway Superficial Epithelia [bulk RNA]

| PRJNA673906 | ENA

Secretory Cells Dominate Airway CFTR Expression and Function in Human Airway Superficial Epithelia [single-cell RNA-seq]

Project description:Secretory Cells Dominate Airway CFTR Expression and Function in Human Airway Superficial Epithelia [single-cell RNA-seq]

| PRJNA673905 | ENA

Long intergenic non-coding RNA linc-SUMF1-2 mediates CFTR-associated transcriptional regulation in human airway epithelia

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-01-31 | GSE110290 | GEO

CF vs CFTR-corrected hiPSC-airway at ALI

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-03-02 | GSE203009 | GEO

Type I and type III interferons drive redundant amplification loops to induce a transcriptional signature in influenza-infected airway epithelia

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-08-30 | E-GEOD-43710 | biostudies-arrayexpress

Repair of airway epithelia requires metabolic rewiring towards fatty acid oxidation

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-01-31 | GSE209686 | GEO

Chronic ?2AR stimulation limits CFTR activation in human airway epithelia.

Project description: Not available

| S-EPMC5916247 | biostudies-literature

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