Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Fruitless decommissions regulatory elements to implement cell-type-specific neuronal masculinization [RNA-Seq]

ABSTRACT: Gene expression profile of neurons comprising most of the central brain courtship circuit in adult male Drosophila brains, with sex and cell-type control populations.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE160930 | GEO | 2021/01/22

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Fruitless decommissions regulatory elements to implement cell-type-specific neuronal masculinization [ATAC-seq]

Project description:Chromatin accessibility of neurons comprising most of the central brain courtship circuit in adult male Drosophila brains, with sex and cell-type control populations.

2021-01-22 | GSE159600 | GEO

Fruitless decommissions regulatory elements to implement cell-type-specific neuronal masculinization.

Project description: Not available

| S-EPMC7924761 | biostudies-literature

Fruitless decommissions regulatory elements to implement cell-type-specific neuronal masculinization

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-01-22 | GSE160931 | GEO

Fruitless decommissions regulatory elements to implement cell-type-specific neuronal masculinization

Project description:Fruitless decommissions regulatory elements to implement cell-type-specific neuronal masculinization

| PRJNA674852 | ENA

Fruitless decommissions regulatory elements to implement cell-type-specific neuronal masculinization [RNA-Seq]

Project description:Fruitless decommissions regulatory elements to implement cell-type-specific neuronal masculinization [RNA-Seq]

| PRJNA674857 | ENA

Fruitless decommissions regulatory elements to implement cell-type-specific neuronal masculinization [ATAC-seq]

Project description:Fruitless decommissions regulatory elements to implement cell-type-specific neuronal masculinization [ATAC-seq]

| PRJNA669853 | ENA

Male-specific Fruitless isoforms have different regulatory roles conferred by distinct zinc finger DNA binding domains

2013-09-30 | GSE50515 | GEO

Male-specific Fruitless isoforms have different regulatory roles conferred by distinct zinc finger DNA binding domains

Project description:Drosophila melanogaster adult males perform an elaborate courtship ritual to entice females to mate. fruitless (fru), a gene that is one of the key regulators of male courtship behavior, encodes multiple male-specific isoforms (FruM). These isoforms vary in their carboxy-terminal zinc finger domains, which are predicted to facilitate DNA binding. By over-expressing individual FruM isoforms in fru-expressing neurons in either males or females and assaying the global transcriptional response by RNA-sequencing, we show that three FruM isoforms have different regulatory activities that depend on the sex of the fly. We identified several sets of genes regulated downstream of FruM isoforms. RNA seqeuncing was performed on mRNA derived from adult male or female heads, for a total of 39 samples. These samples included two wild type genotypes (Berlin and Canton-S), two transheterozygous mutants for fru P1 (Df(3R)P14/Df(3R)fru4-40 and fruw12/ Df(3R)ChaM5), and 3 overexpressing genotypes (fru P1-Gal4: UAS-FruMA, UAS-FruMB, UAS-FruMC). There were at least 3 replicates from biological samples for all sex by genotype combinations.

2013-09-30 | E-GEOD-50515 | biostudies-arrayexpress

The PSI-U1 snRNP interaction regulates male mating behavior in Drosophila

Project description:Alternative pre-mRNA splicing (AS) is a critical regulatory mechanism that operates extensively in the nervous system to produce diverse protein isoforms. Fruitless AS isoforms have been shown to influence male courtship behavior, but the underlying mechanisms are unknown. Using genome-wide approaches and quantitative behavioral assays, we show that the P element somatic inhibitor (PSI) and its interaction with U1 snRNP control male courtship behavior. PSI mutants lacking the U1 snRNP-interacting domain (PSIΔAB mutant) exhibit extended but futile mating attempts. The PSIΔAB mutant results in significant changes in the AS patterns of ~1,200 genes in the Drosophila brain, many of which have been implicated in the regulation of male courtship behavior. PSI directly regulates the AS of at least a third of these transcripts, suggesting that PSI-U1 snRNP interactions coordinate the behavioral network underlying courtship behavior. Importantly, one of these direct targets is fruitless — the master regulator of courtship. Thus, PSI imposes a specific mode of regulatory control within the neuronal circuit controlling courtship, even though it is broadly expressed in the fly nervous system. This study reinforces the importance of AS in the control of gene activity in neurons and integrated neuronal circuits, and provides a surprising link between a pleiotropic pre-mRNA splicing pathway and the precise control of successful male mating behavior.

2016-04-30 | GSE79916 | GEO

Investigation of Drosophila fruitless neurons that express Dpr/DIP cell adhesion molecules

Project description:Drosophila reproductive behaviors are directed by fruitless neurons. A reanalysis of genomic studies shows that genes encoding dpr and DIP Immunoglobulin superfamily (IgSF) members are expressed in fru P1 neurons. We find that each fru P1 and dpr/DIP (fru P1 ∩ dpr/DIP) overlapping expression pattern is similar in both sexes, but there are dimorphisms in neuronal morphology and cell number. Behavioral studies of fru P1 ∩ dpr/DIP perturbation genotypes indicates that the mushroom body functions together with the lateral protocerebral complex to direct courtship behavior. A single-cell RNA-seq analysis of fru P1 neurons shows that many DIPs have high expression in a small set of neurons, whereas the dprs are often expressed in a larger set of neurons at intermediate levels, with a myriad of dpr/DIP expression combinations. Functionally, we find that perturbations of sex hierarchy genes and of DIP-ε change the sex-specific morphologies of fru P1 ∩ DIP-α neurons.

2021-02-23 | GSE162098 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data