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Transcriptome analysis of rice (Oryza sativa cv.TW16) in relation to infection with rice tungro spherical virus (RTSV)

ABSTRACT: Rice tungro disease is caused by the interaction between Rice tungro spherical virus (RTSV) and Rice tungro bacilliform virus. Infection with RTSV alone does not result in any distinctive symptoms in TW16 that is one of RTSV resistant indica rice. To elucidate the basis of asymptomatic response of rice to RTSV at the gene expression level, global gene response in RTSV-infected TN1 was detected by custom microarray. Keywords: time course, virus infection, disease response

ORGANISM(S): Oryza sativa Oryza sativa Indica Group

PROVIDER: GSE16140 | GEO | 2009/12/07

SECONDARY ACCESSION(S): PRJNA122811

REPOSITORIES: GEO

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Transcriptome analysis of rice (Oryza sativa cv.TW16) in relation to infection with rice tungro spherical virus (RTSV)

Project description:Rice tungro disease is caused by the interaction between Rice tungro spherical virus (RTSV) and Rice tungro bacilliform virus. Infection with RTSV alone does not result in any distinctive symptoms in TW16 that is one of RTSV resistant indica rice. To elucidate the basis of asymptomatic response of rice to RTSV at the gene expression level, global gene response in RTSV-infected TN1 was detected by custom microarray. Keywords: time course, virus infection, disease response Comparison between RTSV and mock infected rice. Biological replicates: 3 control (mock), 3 RTSV-infected at each time point, independently grown and harvested at 6 to 15 days after inoculation (DAI). 1 samples prepared with whole plant 5 cm above ground and pooled from 5 plants grown under same conditons.

2010-05-18 | E-GEOD-16140 | biostudies-arrayexpress

Transcriptome analysis of rice (Oryza sativa cv. Taichung Native 1) in relation to infection with RTSV

2009-12-07 | GSE16141 | GEO

Comparative gene expression analysis between virus-susceptible and -resistant rice lines (TW16-1263 and -1029)

2007-10-25 | GSE6125 | GEO

Comparative gene expression analysis between virus-susceptible and -resistant rice lines (TW16-4 and -69)

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Transcription profiling of rice virus-susceptible and -resistant lines (TW16-4 and -69)

Project description:Near-isogenic lines TW16-4 and TW16-69 were developed from the cross between TN1 (recurrent parent susceptible to rice tungro spherical virus (RTSV)) and Utri Merah (donor parent resistant to RTSV). TW16-4 is susceptible to RTSV, whereas TW16-69 is resistant to RTSV. In order to identify genes which are constitutively differentially expressed between TW16-4 and TW16-69, the gene expression in the two lines were compared at three different conditions (healthy, green leafhopper (GLH)-inoculated, and RTSV-inoculated). Experiment Overall Design: Total RNA was isolated from 14-day old plants of TW16-4 and TW16-69 at three different conditions (healthy, GLH-inoculated, and RTSV-inoculated). The levels of gene expression in the two lines of the same condition were compared by microarray. Each experiment was repeated twice.

2008-06-14 | E-GEOD-6124 | biostudies-arrayexpress

Transcription profiling of rice virus-susceptible and -resistant lines (TW16-1263 and -1029)

Project description:Near-isogenic lines TW16-1263 and TW16-1029 were developed from the cross between TN1 (recurrent parent susceptible to rice tungro spherical virus (RTSV)) and Utri Merah (donor parent resistant to RTSV). TW16-1263 is susceptible to RTSV, whereas TW16-1029 is resistant to RTSV. In order to identify genes which are constitutively differentially expressed between TW16-1263 and TW16-1029, the gene expression in the two lines were compared at three different conditions (healthy, green leafhopper (GLH)-inoculated, and RTSV-inoculated). Experiment Overall Design: Total RNA was isolated from 14-day old plants of TW16-1263 and TW16-1029 at three different conditions (healthy, GLH-inoculated, and RTSV-inoculated). The levels of gene expression in the two lines of the same condition were compared by microarray. Each experiment was repeated twice.

2008-06-14 | E-GEOD-6125 | biostudies-arrayexpress

Transcriptome analysis of rice (Oryza sativa cv. Taichung Native 1) in relation to infection with RTSV

Project description:Rice tungro disease is caused by the interaction between Rice tungro spherical virus (RTSV) and Rice tungro bacilliform virus. Infection with RTSV alone does not result in any distinctive symptoms in Taichung Native 1 (TN1) that is one of RTSV susceptive indica rice cultivar. To elucidate the basis of asymptomatic response of rice to RTSV at the gene expression level, global gene response in RTSV-infected TN1 was detected by custom microarray. Keywords: time course, virus infection, disease response Comparison between RTSV and mock infected rice. Biological replicates: 3 control (mock), 3 RTSV-infected at each time point, independently grown and harvested at 6 to 15 days after inoculation (DAI). 1 samples prepared with whole plant 5 cm above ground and pooled from 5 plants grown under same conditons.

2010-05-18 | E-GEOD-16141 | biostudies-arrayexpress

Transcriptome analysis of rice in relation to infection with rice tungro spherical virus (RTSV)

Project description:Near-isogenic lines TW16-4 and TW16-69 were developed from the cross between TN1 (recurrent parent susceptible to RTSV) and Utri Merah (donor parent resistant to RTSV). TW16-4 is susceptible to RTSV, whereas TW16-69 is resistant to RTSV based on a serolog. Keywords: time course, virus infection, disease response

2009-08-31 | GSE14606 | GEO

Comparative analysis of rice plants infected with Rice tungro bacilliform virus

Project description:Rice tungro bacilliform virus (RTBV) is determinant of the rice tungro disease symptom. We compare the gene responses by RTBV infection between RTBV susceptive (TN1) and resistant cultivar (TW16). TN1 infected with RTBV shows the mild stunting and leaf yellowing, although TW16 shows only mild stunting at only early infection stage, and recovers the symptoms. Keywords: virus infection, disease response

2012-12-31 | GSE38150 | GEO

Transcriptome analysis of rice in relation to infection with rice tungro spherical virus (RTSV)

Project description:This SuperSeries is composed of the following subset Series: GSE16140: Transcriptome analysis of rice (Oryza sativa cv.TW16) in relation to infection with rice tungro spherical virus (RTSV) GSE16141: Transcriptome analysis of rice (Oryza sativa cv. Taichung Native 1) in relation to infection with RTSV Refer to individual Series

2010-05-18 | E-GEOD-16142 | biostudies-arrayexpress

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