Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Microglial RNA sequencing from humanized mouse model expressing schizophrenia susceptibility factor C4A

ABSTRACT: Human C4A gene locus is associated with increased schizophrenia risk. In order to investigate this genetic link, we constructed two mouse models that express human C4A or C4B, and one that overexpresses C4A. To investigate whether the isotypic effects of human-C4 gene is observed in the microglial transcriptional profile during adolescence in mice (postnatal day 40) in the medial prefrontal cortex, we conducted a bulk RNA sequencing of microglia. Our analyses showed no significant differentially expressed genes between any two groups.

ORGANISM(S): Mus musculus

PROVIDER: GSE161557 | GEO | 2020/11/17

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Schizophrenia and nicotine proteomics

Project description:Schizophrenia (SCHZ) and tobacco misuse comorbidity is frequently established during adolescence. However, comorbidity markers are still missing. Here, we used an label free quantitative proteomics approach to identify exclusively deregulated proteins in male and female mice modeled to SCHZ with a history of nicotine use during adolescence.

2023-10-24 | PXD043626 | Pride

Alteration of PFC proteome in MK-801 induced schizophrenia-like adolescent mice

Project description:Schizophrenia typically emerges in adolescence or early adulthood. Electrophysiological and several neurochemical changes have linked the GABA deficits to abnormal behaviors induced by NMDAR hypofunction. However, few studies have systematically investigated the molecular basis of GABA deficits, especially during adolescence. To address this issue, we transiently administrated MK-801 to mice on PND 10, which exhibited schizophrenia-relevant deficits in adolescence. Saline treated mice was served as controls. Cortical proteomics was performed on adolescent mice to investigate the proteome alteration.

2022-07-05 | PXD035089 | iProX

Polycomb represses a gene network controlling puberty via modulation of histone demethylase Kdm6b expression

Project description:We report gene expression profiles in the medial basal hypothalami of 28-day-old female rats infected with a lentivirus construct that overexpresses EED and matched controls infected with a GFP-expression construct.

2020-08-09 | GSE102471 | GEO

Bulk RNA-seq of FACS sorted astrocytes upon microglial depletion

Project description:As the professional phagocyte in the central nervous system (CNS), microglia are the primary scavenger removing cell corpses. The failure of microglia in debris clearance influences the normal CNS function. Meanwhile, microglia undergo turnover during the whole lifespan. If dead microglia are not timely removed, accumulated corpses may influence the CNS function. The microglial corpse clearance is hereby crucial for CNS homeostasis. However, the underlying mechanism remains obscure. In this study, we investigated how microglial corpses are removed. We found that microglial corpses are mainly phagocytosed by astrocytes, mediated by C4b opsonization. Then engulfed microglial fragments are degraded in astrocytes via the RUBICON-dependent LC3-associated phagocytosis (LAP), a form of non-canonical autophagy. The interference of the C4b-mediated engulfment and its subsequent LAP disrupt the microglial debris removal and degradation, respectively. Together, we elucidated cellular and molecular mechanisms of microglial debris removal, extending the knowledge on how the CNS homeostasis is maintained.

2022-09-01 | GSE171321 | GEO

Microglial cannabinoid receptor type 1 mediates social memory deficits produced by adolescent THC exposure and 16p11.2 duplication

Project description:Adolescent cannabis use increases the risk for cognitive impairments and psychiatric disorders. Cannabinoid receptor type 1 (Cnr1) is expressed not only in neurons and astrocytes, but also in microglia, which shape synaptic connections during adolescence. Nonetheless, until now, the role of microglia in mediating the adverse cognitive effects of delta-9-tetrahydrocannabinol (THC), the principal psychoactive constituent of cannabis, has been unexplored. Here, we report that adolescent THC exposure produces microglial apoptosis in the medial prefrontal cortex (mPFC), which was exacerbated in the mouse model of 16p11.2 duplication, a representative copy number variation (CNV) risk factor for psychiatric disorders. These effects are mediated by microglial Cnr1, leading to reduction in the excitability of mPFC pyramidal-tract neurons and deficits in social memory in adulthood. Our findings highlight the importance of microglial Cnr1 to produce the adverse effect of cannabis exposure in genetically vulnerable individuals.

2023-10-03 | GSE242892 | GEO

Vibrio parahaemolyticus strain:C4B | isolate:C4B

Project description:Vibrio parahaemolyticus strain:C4B | isolate:C4B Genome sequencing

| PRJNA606186 | ENA

Gene expression profiles of patients with schizophrenia, bipolar disorder and healthy controls

Project description:Schizophrenia (SZ) and bipolar disorder (BD) are severe psychiatric conditions, with a lifetime prevalence of about 1%. Both disorders have a neurodevelopment component, with onset of symptoms occurring most frequently during late adolescence or early adulthood. Genetic findings indicate the existence of an overlap in genetic susceptibility across the disorders. These gene expression profiles were used to identify the molecular mechanisms that differentiate SZ and BP from healthy controls but also that distinguish both from healthy individuals. They were also used to expand an analysis from an experiment that searched molecular alterations in human induced pluripotent stem cells derived from fibroblasts from control subject and individual with schizophrenia and further differentiated to neuron to identify genes relevant for the development of schizophrenia (GSE62105). Brain tissue (frontal cortex) from 30 healthy controls, 29 bipolar disorder patients and 29 schizophrenia patients were analyzed. The reference is an in-house pool of RNA extracted from 15 human cell lines.

2014-10-11 | E-GEOD-62191 | biostudies-arrayexpress

Adolescent Social Isolation Reprograms the Medial Amygdala: Transcriptome and Sex Differences in Reward

Project description:Adolescence is a sensitive window for reward- and stress-associated behavior. Although stress during this period causes long-term changes in behavior in males, how females respond is relatively unknown. Here we show that social isolation stress in adolescence, but not adulthood, induces persistent but opposite effects on anxiety- and cocaine-related behaviors in male vs. female mice, and that these effects are reflected in transcriptional profiles within the adult medial amygdala (meA). By integrating differential gene expression with co-expression network analyses, we identified crystallin mu (Crym), a thyroid hormone binding protein, as a key driver of these transcriptional profiles. Manipulation of Crym specifically within adult meA neurons recapitulates the behavioral and transcriptional effects of social isolation and re-opens a window of plasticity that is otherwise closed. Our results establish that meA is essential for sex-specific responses to stressful and rewarding stimuli through transcriptional programming that occurs during adolescence.

2020-11-30 | GSE146472 | GEO

Microglial RNA sequencing from humanized mouse model expressing schizophrenia susceptibility factor C4A

Project description:Microglial RNA sequencing from humanized mouse model expressing schizophrenia susceptibility factor C4A

| PRJNA678711 | ENA

Gene expression profiles of patients with schizophrenia, bipolar disorder and healthy controls

2014-10-11 | GSE62191 | GEO