RNA sequencing analysis of organoids and their parental tissues

ABSTRACT: We assessed whether organoids maintained the gene expression signatures of their parental healthy tissues and tumors. We performed transcriptomic analysis by bulk RNA sequencing (RNA-seq) across 3 mice, 3 patients and different periods iPSCs. Total RNA of organoids and their parental tissues were extracted at day 7 and day 0, respectively. Total RNA of iPSCs were extracted at day 0, day 1 and day 4. The results show that the organoids are able to replicate the inter-patient heterogeneity, which determines their feasibility on personalized therapy evaluation.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE161928 | GEO | 2020/11/21

REPOSITORIES: GEO