Transcriptomics

Dataset Information

28

0.6% Methamphetamine feeding experiments


ABSTRACT: Methamphetamine can trigger dopamine releasing in human brain, now used as abuse drug. Some studies have shown that specific genes and proteins responded to, methamphetamine, but little is known about the overall “omic” response of organisms to this illicit substance. Here we demonstrate that Drosophila melanogaster has the potential to give us significant insights into evolutionarily conserved responses to methamphetamine. We performed metabolome, proteome, and transciptome profiling with Drosophila treated with methamphetamine. The proteomic profiling revealed responses associated with known physiological problems that occur with methamphetamine usage in mammals. The metabolomic result showed that the metabolite trehalose was decreased significantly after methamphetamine exposure, suggesting an oxidative stress response to this drug. Many of the differential transcribed genes, including detoxification enzymes, had the potential transcription factor-binding motif YY1 associated with their upstream regulatory regions. YY1 is known to be responsive to amphetamines in mammals. Overall design: For each sample, 20 virgin male flies were used to extract the mRNA. Three replicates were produced for each treatments. Two treatments were produced (control VS 0.6% 24 h meth-fed).

REANALYSED by: GSE119128

INSTRUMENT(S): [Drosophila_2] Affymetrix Drosophila Genome 2.0 Array

ORGANISM(S): Drosophila melanogaster  

SUBMITTER: Hongmei LI  

PROVIDER: GSE16198 | GEO |

SECONDARY ACCESSION(S): PRJNA117135

REPOSITORIES: GEO

Similar Datasets

2010-10-30 | E-GEOD-16198 | ArrayExpress
2015-09-03 | E-GEOD-16198 | ExpressionAtlas
2010-03-04 | GSE17665 | GEO
2011-11-15 | E-GEOD-33707 | ArrayExpress
2011-11-15 | GSE33707 | GEO
2012-06-20 | E-GEOD-30305 | ArrayExpress
| GSE111925 | GEO
2004-09-30 | E-GEOD-1711 | ArrayExpress
2017-01-15 | E-MTAB-2843 | ArrayExpress
| GSE46717 | GEO