Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

YTHDF2 inhibits gastric cancer cell growth by regulating FOXC2 signaling pathway

ABSTRACT: We found that YTHDF2 has lower expression in GC tissues and GC cells, and promotes the growth, migration and invasion of GC cells. In addition, the analysis of clinical data found that the expression level of YTHDF2 was closely related to the stage of GC and the survival of patients with GC. RNA-seq showed that overexpression of YTHDF2 significantly reduced protein expression in the FOXC2 (Forkhead box protein C2, FOXC2) signaling pathway.

ORGANISM(S): Homo sapiens

PROVIDER: GSE162004 | GEO | 2021/01/28

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

YTHDF2 inhibits gastric cancer cell growth by regulating FOXC2 signaling pathway

Project description:we found that YTHDF2 has lower expression in GC tissues and GC cells, and promotes the growth, migration and invasion of GC cells. In addition, the analysis of clinical data found that the expression level of YTHDF2 was closely related to the stage of GC and the survival of patients with GC. RNA-seq showed that overexpression of YTHDF2 significantly reduced protein expression in the FOXC2 (Forkhead box protein C2, FOXC2) signaling pathway.

2021-05-05 | GSE168611 | GEO

YTHDF2 inhibits gastric cancer cell growth by regulating FOXC2 signaling pathway

Project description:YTHDF2 inhibits gastric cancer cell growth by regulating FOXC2 signaling pathway

| PRJNA680305 | ENA

YTHDF2 inhibits gastric cancer cell growth by regulating FOXC2 signaling pathway

Project description:YTHDF2 inhibits gastric cancer cell growth by regulating FOXC2 signaling pathway

| PRJNA713007 | ENA

Expression analysis of colorectal cancer cell line SW480 expressing FOXC2

Project description:Investigation of whole genome gene expression level changes in a colorectal cancer cell line SW480 expressing FOXC2, compared to the pBabe control cells. Genes associated with metastasis regulated by FOXC2 in colorectal cancer were analysed. The role of FOXC2 in breast cancer metastasis are further described in Mani SA, Yang J et al. Mesenchyme Forkhead 1 (FOXC2) plays a key role in metastasis and is associated with aggressive basal-like breast cancers. PNAS 2007; 104: 10069-10074 . A six chip study using total RNA recovered from three separate cultures of SW480/pBabe and three separate cultures of SW480/FOXC2. Each chip measures the expression level of 45033 genes from SW480/pBabe or SW480/FOXC2.

2014-07-02 | E-GEOD-58980 | biostudies-arrayexpress

Expression analysis of colorectal cancer cell line SW480 expressing FOXC2

2014-07-02 | GSE58980 | GEO

Differential roles of FOXC2 in the trabecular meshwork and Schlemm’s canal in glaucomatous pathology

Project description:Impaired development and maintenance of Schlemm's canal (SC) are associated with perturbed aqueous humor outflow and intraocular pressure. The angiopoietin (ANGPT)/TIE2 signaling pathway regulates SC development and maintenance, whereas the molecular mechanisms of crosstalk between SC and the neural crest (NC)-derived neighboring tissue, the trabecular meshwork (TM), are poorly understood. Here, we show NC-specific forkhead box (Fox)c2 deletion in mice results in impaired SC morphogenesis, loss of SC identity, and elevated intraocular pressure. Visible-light optical coherence tomography analysis further demonstrated functional impairment of the SC in response to changes in intraocular pressure in NC-Foxc2 -/- mice, suggesting altered TM biomechanics. Single-cell RNA-sequencing analysis identified that this phenotype is predominately characterized by transcriptional changes associated with extracellular matrix organization and stiffness in TM cell clusters, including increased matrix metalloproteinase expression, which can cleave the TIE2 ectodomain to produce soluble TIE2. Moreover, endothelial-specific Foxc2 deletion impaired SC morphogenesis because of reduced TIE2 expression, which was rescued by deleting the TIE2 phosphatase VE-PTP. Thus, Foxc2 is critical in maintaining SC identity and morphogenesis via TM-SC crosstalk.

2023-08-29 | GSE190462 | GEO

YTHDF2 suppresses the plasmablast genetic program and promotes germinal center formation

Project description:Antibody-mediated immunity is initiated by B cell interactions with cognate antigen, followed by differentiation into multiple cell subsets, including plasmablast, memory, and germinal center (GC) cells. B cell differentiation trajectories are determined by specific transcription factors, yet very few mechanisms that determine B cell fate at the early stage of the response have been described. Here, we report an epigenetic mechanism that specifically suppresses the plasmablast genetic program and promotes GC B cell fate commitment. Single-cell RNA-seq analysis of antigen-specific B cells revealed activated B cell precursors at the pre-GC stage that express high levels of RNA binding proteins, including YTHDF2, which enhances the decay of methylated transcripts. Ythdf2-deficient B cells exhibited intact proliferation and upregulation of early activation markers in response to antigenic stimulation, whereas differentiation into GC B cells was blocked. Mechanistically, B cells required YTHDF2 to attenuate the plasmablast genetic program during GC seeding, and key transcripts of plasmablast-related genes, including Irf4 and Xbp1, were methylated and bound by YTHDF2. Modulation of YTHDF2-dependent gene expression by YTHDF1 and YTHDF3 was less pronounced, and no functional redundancy of these paralogs in GC seeding was observed. Collectively, this study reveals a novel epigenetic mechanism that specifically directs appropriate B cell fate commitment through post-transcriptional suppression of gene expression.

2022-04-24 | GSE189819 | GEO

Foxc1 and Foxc2 are necessary to maintain glomerular podocytes

Project description:Foxc1 and Foxc2 are highly expressed in adult podocytes. To bypass embryonic lethality of Foxc1 and Foxc2 KO, mice ubiquitously expressing inducible-Cre (ROSA26-CreERT2) were mated with floxed-Foxc1 and floxed-Foxc2 mice. We used microarrays to detail effects of deletions of Foxc1 and Foxc2 on podocyte gene expression profiles in adult podocyte in vivo and in vitro.

2018-07-25 | GSE117571 | GEO

FOXC2 knockdown in MDA-MB-231 cells

Project description:To determine gene expression changes in vasculogenic mimicry competent human breast cancer cells with loss of FOXC2 we performed RNA-seq of MDA-MB-231 cells with FOXC2 knockdown.

2023-07-26 | GSE232212 | GEO

HMLER cells expressing either FOXC2 or a vector control

Project description:We used microarrays to investigate the transcription profile of FOXC2 expression in a human mammary epithelial cell line. HMLER cells were infected with either a control vector or a retroviral vector expressing FOXC2.

2013-02-15 | E-GEOD-44335 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data