Conventional RNA-seq and 3'end-seq data from HEK293T

ABSTRACT: To inverstigate off-target effects of CRISPR-iPAS, we transfected HEK293TAPA reporter cells with dPguCas13b and gUSE (showed most effective APA-interference) or non-targeting gRNA (gNT) respectively. 3'end-seq and RNA-seq were performed to investigate if the global change on the endogenous PAS usage and gene expression caused by CRISPR-iPAS. Comparing with gNT, dPguCas13b with gUSE showed high specificity.

ORGANISM(S): Homo sapiens

PROVIDER: GSE162305 | GEO | 2022/02/03

REPOSITORIES: GEO