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Vascular integrity is essential for maintaining tissue homeostasis I

ABSTRACT: Purpose: analyze the transcriptomic differences in PBS, LPS and LPS + Riociguat treated whole lung lysate Methods: C57BL6 mice were given PBS, LPS or LPS + Riociguat treatment via trachea instillation. 24h later, homogenize lung and extract mRNA Results: Riociguat can restore LPS-induced lung injury by improving immune environment

ORGANISM(S): Mus musculus

PROVIDER: GSE162328 | GEO | 2021/06/01

REPOSITORIES: GEO

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Vascular integrity is essential for maintaining tissue homeostasis

Project description:Purpose: analyze the transcriptomic differences in PBS and Riociguat treated whole lung lysate Methods: C57BL6 mice were given PBS or Riociguat treatment (i.g.). 24h later, homogenize lung and extract mRNA Results: Riociguat has merely effect on normal mice lung

2022-07-13 | GSE207868 | GEO

Vascular integrity is essential for maintaining tissue homeostasis II

Project description:Purpose: analyze the transcriptomic difference of endothelial cells and pericytes in PBS, LPS or LPS + Riociguat treated lungs Methods: Gucy1a1-EGFP mice were given PBS or LPS treatment via trachea instillation. Vehicle or Riociguat oral administation was performed at every 9 hours. 24h later, lungs were dissociated and endothelial cells and pericytes were isolated by flow cytometry, then directly processed to RNA extraction and library construction Results: Riociguat can restore LPS-induced lung injury by inhibiting pericytes activation and maintain vascular homostatsis

2021-06-01 | GSE162351 | GEO

Vascular integrity is essential for maintaining tissue homeostasis III

Project description:Purpose: analyze the differential changed endothelial cells and pericytes interacton in normal and diseased condition Methods: Pdgfrb-EGFP mice were given PBS or LPS treatment via trachea instillation. 24h later, dissociate lung and isolate endothelial cells and pericytes by flow cytometry, then directly processed to RNA extraction and library construction Results: LPS induced a great transcriptomic change in pericytes than in endothelial cells and NO-sGC pathway was impaired during LPS-induced lung injury

2021-06-01 | GSE162354 | GEO

The mRNA and microRNA (miR) transcriptome of lung neutrophils during S. pneumoniae (SP) pneumonia in wild type (WT) mice [miRNA]

Project description:These studies profiled the expression of mRNAs and microRNAs (miRs) in lung neutrophils in WT mice during S. pneumoniae pneumonia and performed in depth in silico analyses. Lung neutrophils were isolated 24 hours after intratracheal instillation of PBS or S. pneumoniae, and mRNAs and miRs differentially expressed (DE) between S. pneumoniae- and PBS-treated samples were identified using microarrays.

2017-11-01 | GSE97921 | GEO

The mRNA and microRNA (miR) transcriptome of lung neutrophils during S. pneumoniae pneumonia in wild type (WT) mice [mRNA]

2017-11-01 | GSE97920 | GEO

Myeloid PDLIM2 repression as a common causal mechanism of lung disease pathogenesis and susceptibility to infection

Project description:Selective deletion of PDLIM2 in myeloid cells rendered mice more vulnerable to lung injury and mortality after LPS intratracheal instillation, which was associated with the exacerbated activation of pro-inflammation signaling pathways and the more diminished activation of anti-inflammation signaling pathways in the lung, and particularly, in lung macrophages and neutrophils.

2024-02-29 | GSE249800 | GEO

PCR array of inflammatory cytokines and receptors in mouse lungs with NF-kappaB RelA mutant in alveolar epithelial cells during LPS stimulation

Project description:A murine model of RelA mutated throughout the alveolar epithelium was generated. Mice were anesthetized and intratracheally instilled with 50μg LPS into the left lung lobe. Mice were euthanized after 6 hours after instillation, and left lung lobes were collected to isolate RNA. We used SABioscience Mouse Inflammatory Cytokines and Receptors PCR Array to evaluate whether the expressions of lung cytokines and receptors during LPS stimulation are dependent on alveolar epithelial NF-κB RelA or not.

2012-08-03 | GSE38398 | GEO

Data expression in alveolar macrophages induced by lipopolysaccharide in humans

Project description:Rationale: Lipopolysaccharide (LPS) is ubiquitous in the environment. Inhalation of LPS has been implicated in the pathogenesis and/or severity of several lung diseases, including pneumonia, chronic obstructive pulmonary disease and asthma. Alveolar macrophages are the main resident leukocytes exposed to inhaled antigens. Objectives: To obtain insight into which innate immune pathways become activated within human alveolar macrophages upon exposure to LPS in vivo. In seven healthy humans sterile saline was instilled into a lung segment by bronchoscope, followed by instillation of LPS into the contralateral lung. Six hours later a bilateral bronchoalveolar lavage was performed and whole-genome transcriptional profiling was done (Affymetrix HG-U133 Plus 2.0) on purified alveolar macrophages, comparing cells exposed to saline or LPS from the same individuals.

2012-09-15 | E-GEOD-40885 | biostudies-arrayexpress

Transcriptional Analysis of Foxp3+ Regulatory T Cells in Experimental Acute Lung Injury Resolution

Project description:These studies profiled the expression of mRNA in lung and spleen regulatory T cells in Foxp3EGFP mice during lipopolysaccharide-induced lung injury. Baseline, uninjured Lung Treg and Resolving Lung Tregs and Resolving Splenic Tregs were sorted (7 days after intratracheal instillation of LPS for Resolving conditions) and mRNAs differentially expressed (DE) between Control Lung Tregs, Resolving Lung Tregs and Resolving Splenic Tregs samples were identified using microarrays.

2019-02-12 | GSE104088 | GEO

The protective role of hydrogen sulfide and its impact on gene expression profiling in rat model of COPD

Project description:To investigate the role of H2S in Chronic obstructive pulmonary disease (COPD), a rat model of COPD was established by cigarette smoking (CS) and intratracheal instillation of lipopolysaccharide (LPS). Rats were randomly divided into 4 groups: Control, CS+LPS, CS+LPS+NaHS and CS+LPS+propargylglycine (PPG). Transcription profiling of lung tissue comparing Control with CS+LPS, CS+LPS with CS+LPS+NaHS, and CS+LPS with CS+LPS+PPG. The goal was to determine the impact of H2S on gene expression profiling in rat model of COPD.

2021-09-24 | GSE184693 | GEO

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