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Vascular integrity is essential for maintaining tissue homeostasis III

ABSTRACT: Purpose: analyze the differential changed endothelial cells and pericytes interacton in normal and diseased condition Methods: Pdgfrb-EGFP mice were given PBS or LPS treatment via trachea instillation. 24h later, dissociate lung and isolate endothelial cells and pericytes by flow cytometry, then directly processed to RNA extraction and library construction Results: LPS induced a great transcriptomic change in pericytes than in endothelial cells and NO-sGC pathway was impaired during LPS-induced lung injury

ORGANISM(S): Mus musculus

PROVIDER: GSE162354 | GEO | 2021/06/01

REPOSITORIES: GEO

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Vascular integrity is essential for maintaining tissue homeostasis II

Project description:Purpose: analyze the transcriptomic difference of endothelial cells and pericytes in PBS, LPS or LPS + Riociguat treated lungs Methods: Gucy1a1-EGFP mice were given PBS or LPS treatment via trachea instillation. Vehicle or Riociguat oral administation was performed at every 9 hours. 24h later, lungs were dissociated and endothelial cells and pericytes were isolated by flow cytometry, then directly processed to RNA extraction and library construction Results: Riociguat can restore LPS-induced lung injury by inhibiting pericytes activation and maintain vascular homostatsis

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Vascular integrity is essential for maintaining tissue homeostasis I

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FACS-array profiling in retinal endothelial cells from living mouse retinas without pericyte coverage

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Expression data from newborn mouse brain expressing a constitutively active PDGFRb

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2011-06-15 | E-GEOD-29284 | biostudies-arrayexpress

Pericyte sGC signaling dictates tumor vessel formation and tumor microenvironment

Project description:Pericytes and endothelial cells (ECs) are building blocks of blood vessels. While the contributions of ECs to tumor angiogenesis and the tumor microenvironment are well established and multiple drugs that targeting ECs have been developed for clinic use to treat cancers, the underlying mechanisms of pericyte in supporting tumor vessel and in shaping tumor microenvironment remains largely unexplored and no pericyte targeting strategies have been approved yet. This study employs targeted deletion of the NO receptor sGC in pericytes and utilizes single-cell RNA sequencing to elucidate its impact on the tumor microenvironment. The results unveil a disruptive effect on EC-pericyte interactions, subsequently impeding Notch-mediated intercellular crosstalk and prompting extensive transcriptomic reprogramming in both cell types. This vascular alteration further relays to neighboring cancer-associated fibroblasts (CAFs) and tumor-associated macrophages (TAMs) through paracrine signaling, collectively suppressing tumor growth. Importantly, the inhibition of pericyte sGC has limited influence on quiescent vessels but significantly sensitizes angiogenic vessels to anti-angiogenic treatment. In conclusion, this study underscores the vital role of pericytes in governing tumor vessels and the tumor microenvironment, suggesting that targeting pericyte sGC holds promise for enhancing anti-angiogenic therapy.

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Expression data from newborn mouse brain expressing a constitutively active PDGFRb

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Imatinib Disrupts Lymphoma Angiogenesis by Targeting Vascular Pericytes

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Myeloid PDLIM2 repression as a common causal mechanism of lung disease pathogenesis and susceptibility to infection

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PCR array of inflammatory cytokines and receptors in mouse lungs with NF-kappaB RelA mutant in alveolar epithelial cells during LPS stimulation

Project description:A murine model of RelA mutated throughout the alveolar epithelium was generated. Mice were anesthetized and intratracheally instilled with 50μg LPS into the left lung lobe. Mice were euthanized after 6 hours after instillation, and left lung lobes were collected to isolate RNA. We used SABioscience Mouse Inflammatory Cytokines and Receptors PCR Array to evaluate whether the expressions of lung cytokines and receptors during LPS stimulation are dependent on alveolar epithelial NF-κB RelA or not.

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Data expression in alveolar macrophages induced by lipopolysaccharide in humans

Project description:Rationale: Lipopolysaccharide (LPS) is ubiquitous in the environment. Inhalation of LPS has been implicated in the pathogenesis and/or severity of several lung diseases, including pneumonia, chronic obstructive pulmonary disease and asthma. Alveolar macrophages are the main resident leukocytes exposed to inhaled antigens. Objectives: To obtain insight into which innate immune pathways become activated within human alveolar macrophages upon exposure to LPS in vivo. In seven healthy humans sterile saline was instilled into a lung segment by bronchoscope, followed by instillation of LPS into the contralateral lung. Six hours later a bilateral bronchoalveolar lavage was performed and whole-genome transcriptional profiling was done (Affymetrix HG-U133 Plus 2.0) on purified alveolar macrophages, comparing cells exposed to saline or LPS from the same individuals.

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