Dataset Information


Differentially polarized human monocyte-derived macrophages (M0, M1, M2/IL10, M2/IL4, TAM-like)

ABSTRACT: In order to assess whether differences in the transcriptomic profile of human tumor-associated macrophages vs. alveolar macrophages from adjacent non-tumor-tissue (GSE162669) were caused by factors secreted by tumor cells, primary human monocyte-derived macrophages (MDMs) were polarized towards a TAM-like phenotype by cultivating them in tumor cell-conditioned medium. A genome-wide comparison by bulk RNA-Seq confirmed a high similarity of ex vivo TAMs and in vitro polarized TAM-like macrophages. Other polarization schemes (M1: LPS/IFN-gamma, M2: IL10 or IL4) did not lead to similar transcriptomic changes. For details, see Hoppstädter et al., 2021 (doi:10.1016/j.ebiom.2021.103578). Overall design: After differentiation, MDMs were polarized for another 24 hours. To this end, MDM medium was supplemented with 20 ng/ml IFNγ and 100 ng/ml LPS for M1 polarization; either 20 ng/ml IL4 or IL10 for M2 polarization; or left without further supplementation for M0 macrophages. TAM-like macrophages were generated by cultivation in A549 tumor cell-conditioned medium (TCM) supplemented with 20 ng/ml M-CSF. MDM Samples from one female (donor 1) and two male donors (donor 2 and 3) were used for RNA sequencing.

INSTRUMENT(S): Illumina HiSeq 2500 (Homo sapiens)

ORGANISM(S): Homo sapiens  

SUBMITTER: Jessica Hoppstaedter  

PROVIDER: GSE162698 | GEO | 2021-09-25


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<h4>Background</h4>Based on reports on elevated cholesterol levels in cancer cells, strategies to lower cholesterol synthesis have been suggested as an antitumour strategy. However, cholesterol depletion has also been shown to induce tumour-promoting actions in tumour-associated macrophages (TAMs).<h4>Methods</h4>We performed lipidomic and transcriptomic analyses of human lung cancer material. To assess whether the TAM phenotype is shaped by secreted factors produced by tumour cells, primary hum  ...[more]

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