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Inhibition of TGF-β/Smad3 signaling disrupts cardiomyocyte cell cycle progression and EMT-like migration during ventricle regeneration

ABSTRACT: Transcriptome sequencing of SIS3-treated uninjured (5dpf) and injured (2dpt) zebrafish hearts.

ORGANISM(S): Danio rerio

PROVIDER: GSE162820 | GEO | 2021/04/07

REPOSITORIES: GEO

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Gene expression arrays of cardiomyocyte ribosome-associated RNAs during zebrafish heart regeneration

Project description:A transgenic line cmlc2:TRAP was made to express EGFP-fused ribosomal protein L10a (EGFP-L10a) in zebrafish cardiomyocytes. Then ribosome-associated RNAs were immuoprecipitated from uninjured and injured adult cmlc2:TRAP fish to determine the differential expression changes during zebrafish heart regeneration. A nine chip study with cardiomyocyte ribosome associated RNAs purified from three separate isolation of uninjured adult cmlc2:TRAP fish hearts, three separate isolation of 1 day post amupation (dpa) adult cmlc2:TRAP fish hearts, and three separate isolation of 7 dpa adult cmlc2:TRAP fish hearts.

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Inhibition of TGF-β/Smad3 Signaling Disrupts Cardiomyocyte Cell Cycle Progression and Epithelial-Mesenchymal Transition-Like Response During Ventricle Regeneration.

Project description: Not available

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Corrigendum: Inhibition of TGF-β/Smad3 Signaling Disrupts Cardiomyocyte Cell Cycle Progression and Epithelial-Mesenchymal Transition-Like Response During Ventricle Regeneration.

Project description: Not available

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Inhibition of TGF-β/Smad3 signaling disrupts cardiomyocyte cell cycle progression and EMT-like migration during ventricle regeneration

Project description:Inhibition of TGF-β/Smad3 signaling disrupts cardiomyocyte cell cycle progression and EMT-like migration during ventricle regeneration

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Comparison of the gene expression expression profile of activated and resting epicardium

Project description:Zebrafish can regenerate their hearts. As a first response to injury, the epicardium reacts by upregulalion of developmental marker genes. Here we compared in an unbiased manner the expression of the epicardium from uninjured zebrafish hearts and hearts at 3 days postcryoinjury. To label epicardial cells of uninjured hearts we usd pard3:GFP (POon et al 2010) and to label epicardium of the injured heart wt1b:eGFP (Perner et al 2007)

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Gene expression arrays of cardiomyocyte ribosome-associated RNAs during zebrafish heart regeneration

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