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Conjoint Analysis of Metabolome and Transcriptome Reveals the Difference in Flavonoid Biosynthesis in Two Pomelo Cultivars

ABSTRACT: Our study demonstrated the fluctuation of flavonoid biosynthesis in the two pomelo cultivars and laid a theoretical foundation for pomelo breeding to generate fruits with high flavonoid content.

ORGANISM(S): Citrus maxima

PROVIDER: GSE162849 | GEO | 2021/02/01

REPOSITORIES: GEO

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Conjoint Analysis of Metabolome and Transcriptome Reveals the Difference in Flavonoid Biosynthesis in Two Pomelo Cultivars

Project description:Conjoint Analysis of Metabolome and Transcriptome Reveals the Difference in Flavonoid Biosynthesis in Two Pomelo Cultivars

| PRJNA683589 | ENA

Metabolic profiling and transcriptome analysis provide insights into the accumulation of flavonoids in chayote fruit during storage

Project description:Chayote (Sechium edule) fruits are rich in flavonoids, folate, and low-calorie food. However, studies about the flavonoids and regulatory mechanism of flavonoid synthesis in chayote fruits was still unclear. In present study, a transcriptome analysis and metabolite profiling of chayote fruits at three different storage stages were conducted to explore the flavonoid compositions and gene expression associated with flavonoid synthesis. Through the UPLC-MS/MS analysis, a total of 57 flavonoid compounds were detected. Of these, 42 flavonoid glycosides were significantly differential accumulation in chayote fruits at three different storage stages. Many genes associated with flavonoid synthesis were differentially expressed in chayote fruits at three different storage stages through RNA-seq analysis, including structural genes and some TFs. There was a high correlation between RNA-seq analysis and metabolite profiling, and the expression level of candidate genes in the flavonoid synthesis pathway were consistent with the dynamic changes of flavonoids. In addition, one R2R3-MYB transcription factor, FSG0057100, was defined as the critical regulatory gene of flavonoid synthesis. Furthermore, we treated chayote fruits during storage with phenylalanine, and the results show exogenous phenylalanine applications might promote the flavonoid synthesis. Phenylalanine is a effective additive to maintain or improve the total content flavonoids in chayote fruit during storage, can apply the phenylalanine in the postharvest storage of chayote. The above results not only make us better understand the molecular mechanism of flavonoid synthesis in chayote fruits, but also contribute to the promotion and application of chayote products.

2022-10-05 | GSE214641 | GEO

Ectopic expression of FvVND4c promotes secondary cell wall thickening and flavonoid accumulation in Fragaria vesca

Project description:Secondary cell wall thickening (SCW) has a significant effect in the growth and development of plants, as well as in the resistance to various biotic and abiotic stresses. It is regulated by a multilevel transcriptional regulatory network, in which VASCULAR-RELATED NAC-DOMAINs (VNDs) act as key regulators. Lignin is an important component of SCW, it has a cooperative regulation with the biosynthesis of flavonoids which also originate from phenylpropanoid pathway. However, there are few studies on SCW thickening and flavonoid biosynthesis in flesh fruits. We want to investigate the role of FvVNDs on cell wall and fruit color development in Fragaria vesca.

2023-05-01 | GSE225542 | GEO

Effects of flavonoid diet intervention on intestinal flora and stress state in cancer patients

Project description:Intervention group:High flavonoid content fruit and vegetable diet guidance;Control group:No Primary outcome(s): Flavonoid markers;Salivary cortisol;Blood cortisol;Gut microbiota;Mental Health Assessment Questionnaire;Fecal short chain fatty acids;Changes in defecation habits and traits Study Design: Parallel

| 2717029 | ecrin-mdr-crc

Transcriptome analysis in flavonoid overaccumulating and lacking Arabidopsis.

Project description:TranscriptoTranscriptome profiling using DNA microarrays of the aerial parts of the wild-type and other plants was conducted to examine if either MYB overexpression or flavonoid overaccumulation is responsible for the expression of stress-related genes involved in both the biotic and abiotic stress response. Comparative transcriptome analysis using DNA microarray in flavonoid overaccumulating and lacking Arabidopsis.

2013-09-27 | E-GEOD-51215 | biostudies-arrayexpress

Global gene expression analysis of resistant and susceptible apple cultivars challenged with Penicillium expansum

Project description:Blue mold, caused by Penicillium expansum, is responsible for postharvest losses of apple fruit, and threatens human health through production of the potent mycotoxin patulin. No major gene(s) providing resistance have as yet been identified, but recent studies indicate a quantitative control of the disease. An AryANE chip covering 60K apple transcripts was used to identify possible candidate gene(s) that are differentially regulated between resistant and susceptible cultivars upon P. expansum infection. Induction of cell wall related gene (PGIP1), and three genes involved in the â€˜down-streamâ€™ flavonoid biosynthesis pathway (CHS, FLS and LDOX), shows the fundamental role of cell wall as an important barrier, and contents of polyphenolic compounds of fruits as a quantitative components in enhancing disease resistance to blue mold. Moreover, exogenous application of Jasmonic acid hormone enhanced the defense mechanism in fruits. This is the first report linking Jasmonic acid and activation of cell wall and flavonoid pathway genes in apple fruit resistance to blue mold. Results provide an initial categorization of genes that are potentially involved in the resistance mechanism, and should be useful for developing tools for gene marker-assisted breeding of apple cultivars with an improved resistance to blue mold. SUBMITTER_CITATION: Ahmadi-Afzadi, M., Orsel Baldwin, M., Pelletier, S., Cournol, M., Proux-Wéra, E., Nybom, H., Renou, J.-P. (2018). Genome-wide expression analysis suggests a role for jasmonates in the resistance to blue mold in apple. Plant Growth Regulation, 85 (3), 375-387. , DOI : 10.1007/s10725-018-0388-2

2018-06-01 | GSE99966 | GEO

RNA-Seq analysis of the flavonoid-deficient Arabidopsis line, tt4-11

Project description:This is the first analysis of an Arabidopsis flavonoid mutant line by RNA-Seq, specifically, the chalcone synthase-deficient line, tt4-11. There is one other publically-available microarray dataset for a related allele, tt4-3 (Nakabayashi et al., Plant J. 2014). The data provide evidence for alterations in circadian clock and clock-controlled gene expression in the absence of chalcone synthase protein and/or flavonoid endproducts.

2022-02-20 | GSE196636 | GEO

UV Radiation gene profiling in Maize Genotypes with Varying Flavonoid Content

Project description:Microarray hybridization was used to assess acclimation responses to four UV regimes by near isogenic Zea mays lines varying in flavonoid content.

2010-06-05 | E-GEOD-573 | biostudies-arrayexpress

Dissecting the Variations of Ripening Progression and Flavonoid Metabolism in Grape Berries Grown under Double Cropping System

Project description:A double cropping system has been commercially adopted in subtropical regions in southern China, where there is abundant sunshine and heat resources. In this viticulture system, the first growing season normally starts as a summer cropping cycle; then, the vine is pruned and forced by hydrogen cyanamide, resulting in a second crop in January of the next year. Due to climate differences between the two growing seasons, flavonoid content and composition varies greatly. In this study, changes in the transcriptome of flavonoid-associated pathways were compared in berries grown under the double cropping system; in addition, the accumulation of flavonoid compounds was compared. Specific alterations in MYB transcription factors occurred in winter cropping berries around veraison. Then, the winter cropping cycle distinctly induced the flavonoid metabolic pathways while triggering the ripening-associated pathways. Notably, the climate conditions in winter cropping positively affected flavonoid biosynthesis, while the summer season took a major toll on anthocyanin accumulation. In addition, the three classes of flavonoid compounds responded differently to the changing climate; the anthocyanins and flavonols were promoted several fold, whereas no consistent increase was found for flavan-3-ols. Conclusively, flavonoid biosynthesis in grapes grown under a double cropping system showed seasonal or climatic-specific accumulation patterns.

2017-10-01 | GSE103226 | GEO

UV Radiation gene profiling in Maize Genotypes with Varying Flavonoid Content

Project description:Microarray hybridization was used to assess acclimation responses to four UV regimes by near isogenic Zea mays lines varying in flavonoid content. Keywords: other

2003-08-04 | GSE573 | GEO

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