Genomics

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High-throughput single cardiomyocyte transcriptomics identify novel gene programs underlying the normal developmental process of terminal cardiomyocyte differentiation


ABSTRACT: It is generally accepted that G2M cell cycle arrest occurs around birth (P3 in mice) as a natural process of cardiomyocyte (CM) terminal differentiation resulting in binucleation/polyploidization that renders the mammalian heart non-regenerative after damage. Mechanisms underlying this postnatal switch in CM proliferation remain unclear. Herein, we developed a novel approach combining fluorescence-activated cell sorting (FACS) with single cell RNA sequencing (scRNA-seq) of fixed CMs and specifically identified transcription factors (TFs) involved in G2M cell cycle completion of mouse CMs.

ORGANISM(S): Mus musculus

PROVIDER: GSE162959 | GEO | 2023/03/16

REPOSITORIES: GEO

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