Bulk transcriptome sequencing (bulkRNA-seq) of peripheral blood leukocytes (PBLs) in the bacterial meningitis (BM) of children

ABSTRACT: Purpose: Profiling the bulk transcriptomes of PBLs in sepsis-developed BM progression. Methods: The total RNA of PBLs from BM patients were extracted, and constructed into cDNA library. Raw data of mRNA profiles were sequenced by paired-end strategy. Gene-counts were generated through handling sequencing data with the combined workflow of UMI-tools, STAR, Subread package and Samtools. Results: Successfully acquirng multiple bulk-transcriptomic profiles of PBLs from BM patients in different sepsis conditions.

ORGANISM(S): Homo sapiens

PROVIDER: GSE163196 | GEO | 2020/12/15

REPOSITORIES: GEO