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ATACseq Analysis CD4+ T cells response to isoalloLCA treatment in vitro

ABSTRACT: We examine the chromatin landscape of murine CD4+ T cell cultured under Th0 condition (anti-CD3; anti-CD28; IL-2), treated with DMSO, isoalloLCA (20 uM) for 48 hrs, We compare cells from wild-type control mice and Nr4a1-deficient mice

ORGANISM(S): Mus musculus

PROVIDER: GSE163350 | GEO | 2021/01/06

REPOSITORIES: GEO

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RNAseq Analysis CD4+ T cells response to isoalloLCA treatment in vitro

Project description:We examine the transcription response of murine CD4+ T cell cultured under Th0 condition (anti-CD3; anti-CD28; IL-2), treated with DMSO, isoalloLCA (20 uM) for 48 hrs, or in the presence of TGFbeta (0.25 ng/mL). We compare the differential genes regulated by isoalloLCA and TGFbeta.

2021-01-06 | GSE163349 | GEO

Expression profiling comparisons of human CD4+ T cells treated with RORgt inhibitors

Project description:The aim of this study was to identify differential gene expression resulting from the inhibition of RORgt in human CD4+ T cells. Human CD4+ T cells were cultured with anti-CD3/anti-CD28 mAb for 20 to 60 hours concurrent with exposure to Vehicle (DMSO), TMP778 (0.2 uM) or TMP776 (0.2 uM). All samples were then processed for RNA extraction, labeling, and hybridization to Affymetrix arrays.

2014-03-07 | E-GEOD-53455 | biostudies-arrayexpress

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Project description:The aim of the project is to determine the binding sites of Ikaros in CD4+ T cells. To perform this, CD4 naive T cells (CD4 TN; d0) either rested or activated with anti-CD3+anti-CD28 Abs for 1 day (Th0 d1) were subjected to a ChIP using anti-Ikaros Ab followed by high throughput sequencing.

2021-04-26 | GSE157811 | GEO

Transcription profiling of mouse spleen CD4+ CD44Low CD62LHigh cells stimulated with anti-CD3 or anti-CD28 in the presence or absence of hTGFb-1 and mouse IL-6

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2008-12-21 | E-TABM-583 | biostudies-arrayexpress

Retinoic Acid Induced Transcriptional Repressor HIC1 is Required for Suppressive Function of Human Induced Regulatory T cells [RNA-Seq 2]

Project description:Human CD4 positive T cells were isolated from cord blood using CD4 positive isolation kit from Dynal. Cells were activated with plate bound anti-CD3 and soluble anti-CD28 in presence (iTreg) or absence (Th0) of IL2, TGF beta and ATRA. The cells were harvested at 0, 0.5, 1, 2, 4, 6, 12, 24, 48, and 72 hours.

2018-02-21 | GSE90569 | GEO

RNA-SEQ analysis of CD4+ T cells in presence or absence of Ikaros.

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Effect of acute pyrvinium pamoate treatment on gene expression of CD4+ T cells during early activation.

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2023-08-16 | GSE229494 | GEO

Effect of costimulation on microRNAs levels in human naïve CD4 T cells

Project description:microRNA transcriptional profiling of human naïve CD4 T cells comparing cells that saw no activation, only anti-CD3 or anti-CD3 and anti-CD28 to identify microRNAs specifically upregulated by CD28-mediated costimulation. Three conditions experiment. PBS, anti-CD3, anti-CD3 + anti-CD28 all compared to a reference sample. Biological triplicates, independently extracted and activated.

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Effect of costimulation on microRNAs levels in human naïve CD4 T cells

2012-02-25 | GSE36071 | GEO

TWS119 treatment of human memory T cells

Project description:We want to identify gene expression pattern in memory cells in response to Wnt pathway activation human memory T cells from peripheral blood from three healthy individuals were stimulated with anti-CD3/CD28 in the presence and absence of TWS119, a Wnt pathway activator. DMSO was added as control vehicle.

2016-12-15 | GSE59400 | GEO

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