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Chip-seq, ATAC-seq, and RNA-seq of murine P14 T cells WT or FOXO1 KO, responding in vivo to LCMV-ARM

ABSTRACT: P14 CD8+ murine T cells were adoptively transferred to C57BL/6 hosts and infected with LCMV-ARM. At indicated times post-infection (see sample name), P14 T cells of the indicated cell-surface phenotype were isolated and sujectected to ChIP-seq, RNA-seq or ATAC-seq.

ORGANISM(S): Mus musculus

PROVIDER: GSE163723 | GEO | 2021/02/19

REPOSITORIES: GEO

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Chip-seq, ATAC-seq, and RNA-seq of murine P14 T cells WT or FOXO1 KO, responding in vivo to LCMV-ARM

Project description:Chip-seq, ATAC-seq, and RNA-seq of murine P14 T cells WT or FOXO1 KO, responding in vivo to LCMV-ARM

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Single-cell sequecing of LCMV-specific WT and HIF-1a-deficient CD8 T cells isolated from LCMV-Clone13 infected host mice

Project description:To determine the role of hypoxia-inducible factor 1 alpha (HIF-1a) in exhausted T cells, we crossed Hif1a-fl/fl Cd4-Cre mice to tdTomato+ P14 animals and adoptively co-transferred equal numbers of WT and HIF-1a-deficient P14 CD8+ T cells into chronically infected WT host mice. 21 days post infection, we retrieved GFP+ (WT) and tdTomato+ (HIF-1a-deficient) donor P14 T cells by FACS sorting and subjected CITE-seq antibody-barcoded WT and HIF-1a-deficient CD8+ P14 T cells to multiplexed scRNA sequencing analysis.

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RNA-seq of P14 CD8 T cells during acute LCMV infection

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Project description:The PI3K/Akt signaling pathway impacts various aspects of CD8 T cell homeostasis, such as effect versus memory cell differentiation, during viral infection. We used microarrays to determine which downstream molecules were affected and what other signaling pathways were interconnected with the Akt pathway by constitutive activation of Akt in LCMV-infected CD8 T cells. Splenocytes from naive P14/WT or P14/Akt mice were stained with anti-CD8 and anti-Ly5.1, and CD8 T cells were sorted using a FACSAria II instrument. Purified Ly5.1+ CD8 T cells from P14/WT or P14/Akt mice were transferred into B6 mice, which were subsequently infected with LCMV Armstrong. At day 8 post infection, splenocytes were stained with anti-CD8, anti-Ly5.1, anti-KLRG1, and anti-CD127. Following staining, short-lived effector cells (SLECs) and memory precursor effector cells (MPECs) were sorted using the FACSAria II instrument; the purity of the sorted cells was >95%. A total of 5 samples were analyzed, including WT naive, WT SLEC, WT MPEC, Akt naive and Akt SLEC.

2012-03-01 | E-GEOD-36168 | biostudies-arrayexpress

H3K27Ac ChIP-seq of P14 CD8 T cells during acute LCMV infection

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RNA-seq of LCMV GP33-specific CD8 TCR transgenic cells (P14) primed at the onset versus in the midst of persistent LCMV-Clone 13 viral infection

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ATAC-seq of P14 CD8 T cells during acute LCMV infection

Project description:ATAC-seq of P14 CD8 T cells during acute LCMV infection

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RNA-seq of P14 CD8 T cells during acute LCMV infection

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2015-05-20 | E-GEOD-63472 | biostudies-arrayexpress

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