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Chromatin accessibility analysis of CNS4-sufficient and defincient regulatory T cells, Treg cell precursors, and mature and semimature CD4 single positives

ABSTRACT: We have discovered that deletion of a conserved regulatory element upstream of the Foxp3 gene, termed CNS4, results in the abrogation of IL-2 mediated induction of Foxp3 expression during regulatory T cell development. However, the mice still harbor a reduced but functional population of Treg cells. To understand potential compensatory mechanisms driving the differentiation and expansion of regulatory T cells in the absence of CNS4, we profiled CNS4-deficient and -sufficient regulatory T cells, as well as related cell types, from the thymuses of male mice by ATAC-sequencing.

ORGANISM(S): Mus musculus

PROVIDER: GSE163811 | GEO | 2021/02/28

REPOSITORIES: GEO

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A distal Foxp3 enhancer enables interleukin-2 dependent thymic Treg cell lineage commitment for robust immune tolerance

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