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Differential expression of Aedes aegypti salivary gland transcriptome upon blood feeding

ABSTRACT: We have developed an oligoGEArray with 434 genes from Ae. aegypti salivary gland. OligoGEArray was customized with the salivary gland genes chosen from the transcriptome source published by Ribeiro et al (2007). Oligonucleotides (60bp) were designed and array were manufactured by SABiosciences. Using this OligoGEArray, we analyzed the differential expression of salivary trancritptome upon blood feeding.

ORGANISM(S): Aedes aegypti

PROVIDER: GSE16392 | GEO | 2009/12/31

SECONDARY ACCESSION(S): PRJNA116279

REPOSITORIES: GEO

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Differential expression of Aedes aegypti salivary gland transcriptome upon blood feeding

Project description:We have developed an oligoGEArray with 434 genes from Ae. aegypti salivary gland. OligoGEArray was customized with the salivary gland genes chosen from the transcriptome source published by Ribeiro et al (2007). Oligonucleotides (60bp) were designed and array were manufactured by SABiosciences. Using this OligoGEArray, we analyzed the differential expression of salivary trancritptome upon blood feeding. Salivary glands were dissected from 1, 3, 24 and 48 hours post fed (hpf) and unfed Ae. aegypti. Total RNA were extracted and the differential expression of transcriptome were analysed. Quantitative Real-Time PCR was performed on selected genes to validate the OligoGEArray data.

2010-06-24 | E-GEOD-16392 | biostudies-arrayexpress

Proteomic analysis of salivary glands of female Aedes aegypti Linn

Project description:Aedes aegypti is a major vector for dengue, chikungunya and yellow fever. Though salivary gland plays a crucial role in transmission of virus and vector life cycle, there is no global and unbiased published report on salivary gland proteome of Ae. aegypti. In this study, we have carried out mass spectrometry based proteomic analysis of salivary gland from adult female Ae. aegypti mosquitoes. By fractionating the proteins isolated from salivary gland on SDS-PAGE and analyzing the in-gel digested bands on high resolution mass spectrometry, we identified 1,205 proteins. This is by far the largest catalogue of salivary gland proteome of Ae. aegypti. These proteins were then assigned molecular functions and biological processes. Several immunity related pathways were found to be enriched in salivary gland. In addition, subset of proteins was predicted to secretory in nature and may play an important role during blood feeding. This study provides a useful resource of proteins expressed in salivary gland of Ae. aegypti female mosquitoes and will aid in biomedical research focused on development of transmission blocking vaccine.

2017-02-28 | PXD002468 | Pride

Gene expression profiling in wMelPop-infected Aedes aegypti

Project description:Whole genome transcriptional profiling comparing Ae. aegypti infected with Wolbachia sp. wMelPop (PGYP1) to an uninfected Ae. aegypti control line (PGYP1.tet). The objective of the experiment was to identify genes that may be involved in the life shortening phenotype associated with wMelPop infection.

2009-08-03 | GSE17469 | GEO

Gene expression profiling in wMelPop-infected Aedes aegypti

2010-05-15 | E-GEOD-17469 | biostudies-arrayexpress

Aedes aegypti RNA seq Insecticide resistance LECA Grenoble

Project description:RNA seq comparison of different Ae. Aegypti strains obtained by selection with various insecticides.

2013-09-01 | E-MTAB-1635 | biostudies-arrayexpress

Analysis of the Aedes aegypti transcriptome during flavivirus infection

Project description:Microarray analysis on days 1, 2 and 7 post-infection with dengue, yellow fever and West Nile virus in Aedes aegypti Rockefeller strain mosquitoes RNA was purified and hybridized with Nimblegen X4 microarray chips using 81-mer probes designed from 18,000 open reading frames (ORF) found in the Ae. aegypti genome, with 2 different probes per ORF

2013-03-31 | GSE28208 | GEO

Aedes aegypti

Project description:Aedes aegypti salivary gland transcriptome from male and female adult organisms

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Project description:Differential expression of Aedes aegypti salivary gland transcriptome upon blood feeding

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The Antiviral Small-Interfering RNA Pathway Induces Zika Virus Resistance in Transgenic Aedes aegypti

Project description:We have generated transgenic Ae. aegypti that express a ZIKV-specific inverted repeat RNA intended to trigger the mosquito siRNA anti-viral immune pathway. To confirm that the transgene is expressed and processed, we performed small RNA sequencing on both Higgs White Eye (HWE) & anti-NS3/4A-ZIKV transgenic Ae. aegypti 24 hours post-bloodmeal

2020-08-26 | GSE156825 | GEO

The effect of permethrin resistance on Aedes aegypti transcriptome following ingestion of Zika virus infected blood

Project description:Aedes aegypti (L.) is the primary vector of many emerging arboviruses. Insecticide resistance among mosquito populations is a consequence of the application of insecticides for mosquito control. We used RNA-sequencing to compare transcriptomes between permethrin resistant and susceptible strains of Florida Ae. aegypti in response to Zika virus infection. A total of 2,459 transcripts were expressed at significantly different levels between resistant and susceptible Ae. aegypti. Gene ontology analysis placed these genes into 7 categories of biological processes. The 863 transcripts were expressed at significantly different levels between two strains (up/down regulated) more than 2-fold. Quantitative real-time PCR analysis validated Zika-infected response, and suggested a highly overexpressed P450, with AAEL014617 and AAEL006798 as potential candidates for the molecular mechanism of permethrin resistance in Ae. aegypti. Our findings indicated that most detoxification enzymes and immune system enzymes altered their gene expression between the two strains of Ae. aegypti in response to Zika virus infection. Understanding the interactions of arboviruses with resistant mosquito vectors at the molecular level allows for the possible development of new approaches in mitigating arbovirus transmission. This information sheds light on Zika-induced changes in the insecticide resistance of Ae. aegypti with implications for mosquito control strategies.

2018-08-22 | GSE118858 | GEO

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