3D-Bioprinting allows the establishment of long-term 3D culture model for Chronic Lymphocytic Leukemia cells

ABSTRACT: Studying primary Chronic Lymphocytic Leukemia cells in vitro is very challenging due to their short survival in culture, and also due to the fact that traditional two-dimensional in vitro models lack cellular and spatial complexity present in vivo. Based on these considerations, we exploited three-dimensional (3D) bioprinting to advance in vitro models for CLL. Through RNA sequencing (RNAseq) analysis, we observed a consistent difference in gene expression profile between 2D and 3D samples, indicating a different behavior of the cells in the two different culture settings.

ORGANISM(S): Homo sapiens

PROVIDER: GSE163977 | GEO | 2021/04/21

REPOSITORIES: GEO