Gene expression profile of primary human monocyte subsets

ABSTRACT: Human primary monocytes are composed of a minor, more mature CD16+(CD14low/neg) population and a major CD16neg(CD14+) subset. The specific functions of CD16+ vs. CD16neg monocytes in steady state or inflammation remain poorly understood. In previous work we found that IL-12 is selectively produced by the CD16+ subset in response to the protozoan pathogen, Toxoplasma gondii. Here we demonstrated that this differential responsiveness correlates with the presence of an IFN-induced transcriptional signature in CD16+ monocytes already at baseline. Consistent with this observation, we found that in vitro IFN-γ-priming overcomes the defect in IL-12 response of the CD16neg subset. We have anayzed the gene expression profile of primary human CD16+ and CD16neg monocyte subsets cultured for without or with IFN-gamma

ORGANISM(S): Homo sapiens

PROVIDER: GSE164346 | GEO | 2021/01/07

REPOSITORIES: GEO