Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Asc-1 regulates white versus beige adipocyte fate in a subcutaneous stromal cell population

ABSTRACT: Here we provide scRNAseq data from the stromal vascular fraction of adult (8 weeks) and adolescent (2 weeks) murine inguinal subcutaneous white adipose tissue. This allows studying cellular composition and cellular heterogeneity within subcutaneous fat.

ORGANISM(S): Mus musculus

PROVIDER: GSE164350 | GEO | 2021/01/07

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

A non-cell autonomous mechanism of beige adipocyte renaissance in subcutaneous adipose tissue

Project description:Two types of UCP1 positive cells-brown and beige adipocytes exist in mammals. Beige adipocytes are very plastic, and can be dynamically regulated by environment.Beige adipocytes formed postnatally in subcutaneous inguinal white adipose tissue (iWAT) lost thermogenic gene expression and multilocular morphology at adult stage, but cold could restore their “beigeing” characteristics, a phenomenon termed as beige adipocyte renaissance. Our results showed that beige cell maintenance and renaissance in adult mice were regulated by cAMP and HDAC4 signaling in white adipocytes non-cell autonomously. Genetic modulations of various components of this cAMP-HDAC4 cascade (e.g. LKB1) led to persistent browning and reduced adiposity independent of thermogenesis. To further study the mechanisms of beige adipocytes maintenance, we performed RNA-seq with samples from inguinal white adipose tissues of WT, AdipoqCre LKB1 F/F, and AdipoqCre LKB1 F/F; HDAC4 F/F mice.Our studies will move the beige adipocyte field forward and attract clinical applications to target beige adipocyte renaissance.

2018-02-14 | GSE85335 | GEO

Asc-1 regulates white versus beige adipocyte fate in a subcutaneous stromal cell population.

Project description: Not available

| S-EPMC7952576 | biostudies-literature

Molecular basis of beige adipocyte maintenance

Project description:Mature adipocytes from Inguinal WAT of CL316,243-treated Ucp1Cre/+;Rosa26-LSL-GFP mice were isolated and GFP+ adipocytes were collected and subjected to transcriptome profiling by RNA-seq.

2016-08-25 | E-MTAB-3978 | biostudies-arrayexpress

Ablation of adipocyte BCL6 enhances subcutaneous adiposity and insulin sensitivity [ChIP-seq]

Project description:In adipocyte-specific knockout mice (Bcl6AKO), we found that Bcl6 deletion results in strikingly increased inguinal but not perigonadal adipocyte size and tissue mass in addition to marked insulin sensitivity. Genome-wide DNA binding and RNA expression analyses revealed that BCL6 controls gene networks involved in cell growth and fatty acid biosynthesis. Thus, our studies identify BCL6 as a negative regulator of subcutaneous adipose tissue expansion and metabolic health.

2019-02-26 | GSE113407 | GEO

Ablation of adipocyte BCL6 enhances subcutaneous adiposity and insulin sensitivity [RNA-seq]

2019-02-26 | GSE113406 | GEO

Transcriptomic profiling of subcutaneous white adipose tissue with selective ablation of Bmal1 at ambient and cold condition

Project description:Mice with beige fat-selective ablation of Bmal1 were generated by crossing Bmal1fl/fl with Prrx1-Cre transgenics. Inguinal subcutaneous adipsoe tissues were collected under ambient temperature at 220C or cold acclimations at 10oC for two weeks.

2021-08-31 | GSE183000 | GEO

Asc-1 regulates white versus beige adipocyte fate in a subcutaneous stromal cell population

Project description:Asc-1 regulates white versus beige adipocyte fate in a subcutaneous stromal cell population

| PRJNA690085 | ENA

Naa10p Inhibits Beige Adipocyte-mediated Thermogenesis through N-α-acetylation of Pgc1α

Project description:We reported that both conventional and adipose-specific Naa10p deletions in mice result in increased energy expenditure, thermogenesis, beige adipocyte differentiation and activation. Mechanistically, Naa10p acetylates the N-terminus of Pgc1α and prevents it from interacting with Ppar to activate key genes, such as Ucp1, involved in beige adipocyte function. We used microarrays to profile the gene expression changes by Naa10p KO in inguinal white adipose tissues (iWATs) derived from mice fed with high fat diet for 15 weeks.

2019-08-16 | GSE133865 | GEO

RNA-Seq of subcutaneous inguinal white fat of Hlx transgenic mice and littermate controls.

Project description:RNA-Seq of subcutaneous inguinal white fat of Hlx transgenic mice and littermate controls.

2017-08-11 | GSE78143 | GEO

Single cell functional genomics reveals plasticity of subcutaneous white adipose tissue (WAT) during early postnatal development

Project description:We performed a large-scale single cell transcriptomic (scRNA-seq) and epigenomic (snATAC-seq) characterization of cellular subtypes (adipose stromal cells (ASC) and adipocyte nuclei) during inguinal WAT (subcutaneous; iWAT) development in mice, capturing the early postnatal period (postnatal days (PND) 06 and 18) through adulthood (PND56).

2021-09-08 | GSE180589 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data