Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification of Nfia-target genes in mid-gestational NS/PCs

ABSTRACT: The purpose of this study is to identify Nfia-target genes, and then clarify how Nifa induces gliogenic potential during brain development.

ORGANISM(S): Mus musculus

PROVIDER: GSE164503 | GEO | 2021/04/15

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Identification of Nfia-target genes in mid-gestational NS/PCs

Project description:Identification of Nfia-target genes in mid-gestational NS/PCs

| PRJNA691023 | ENA

Single cell RNA-seq analysis of feeder-free iPSC-derived gliogenic NS/PCs and neurogenic NS/PCs

Project description:We performed single cell RNA sequencing to analyze the transcriptional profile of gliogenic NS/PCs and neurogenic NS/PCs derived from the same parental feeder-free iPSCs.

2020-12-09 | GSE138302 | GEO

Sequential expression profile of feeder-free iPSC-derived gliogenic NS/PCs by RNA-seq

Project description:We performed RNA sequencing to profile the temporal dynamics of gene expression during gliogenic NS/PC differentiation from human iPSCs.

2020-12-09 | GSE138301 | GEO

Mouse ESC-derived NSPCs NFIA OE (miRNA)

Project description:Since the NFI transcription factors have been shown to be key regulators of gliogenesis, we utilized this pathway to identify miRNAs involved in the regulation of the neurogenesis-to-gliogenesis switch by neural stem/progenitor cells (NSPCs). We focused on miRNAs with expression levels that were differentially regulated downstream of NFIA, and established a mouse embryonic stem cell (ESC) line that expresses NFIA in a doxycycline (Dox)-dependent manner. NFIA-overexpressing (OE) and control NSPCs (neurospheres) derived from ESCs were purified from their mixed cultures (primary neursphsres (PNs) or secondary neurospheres (SNs) ) by fluorescence activated cell sorting and subjected to miRNAarray analysis.

2015-08-01 | E-GEOD-70128 | biostudies-arrayexpress

Mouse ESC-derived NSPCs NFIA OE (GE)

2015-08-01 | E-GEOD-70130 | biostudies-arrayexpress

Single cell RNA-seq analysis of feeder-free iPSC-derived gliogenic NS/PCs and neurogenic NS/PCs

Project description:Single cell RNA-seq analysis of feeder-free iPSC-derived gliogenic NS/PCs and neurogenic NS/PCs

| PRJNA575368 | ENA

Region-specific DNA-binding properties of NFIA in the adult mouse brain

Project description:The goal of this study is to profile NFIA DNA-binding properties in the adult mouse brain. We performed chromatin immunoprecipitation of NFIA in the hippocampus and olfactory bulb of wildtype mice, and samples were subjected to sequencing. We find that NFIA preferentially binds DNA in the hippocampus but not in the olfactory bulb as evidenced by the distinct lack of NFIA binding peaks in the olfactory bulb. Mass spectrometry results suggested that NFIA has a significantly higher binding affinity for NFIB in the olfactory bulb, potentially blocking NFIA’s ability to bind DNA. Virally induced siRNAs against NFIB or scramble were injected into the olfactory bulb of adult wildtype mice to knock down NFIB. We performed chromatin immunoprecipitation of NFIA in the olfactory bulb injected with siRNA-NFIB or siRNA-scramble. Subsequent sequencing revealed an increase of NFIA binding in the olfactory bulb upon the depletion of NFIB as compared to the siRNA-scramble and wildtype controls.

2020-02-28 | GSE146064 | GEO

Regional gene expression profiling upon loss of NFIA in adult astrocytes

Project description:The goal of this study is to determine how the loss of the transcription factor NFIA affects the molecular profiles of adult astrocytes from four brain regions. We performed RNA-sequencing on control and NFIA knockout (KO) astrocytes from the olfactory bulb, hippocampus, cortex, and brainstem, and analyzed the molecular signatures of NFIA KO astrocytes compared to control in each brain region.

2020-02-27 | GSE146000 | GEO

Mouse ESC-derived NSPCs NFIA OE (miRNA)

2015-08-01 | GSE70128 | GEO

Mouse ESC-derived NSPCs NFIA OE (GE)

2015-08-01 | GSE70130 | GEO