Transcriptomics

Dataset Information

0

A Growth Factor-Expressing Macrophage subpopulation orchestrates regenerative inflammation via GDF-15


ABSTRACT: Muscle regeneration is the result of the concerted action of multiple cell types driven by the temporarily controlled phenotype switches of infiltrating monocyte-derived macrophages. Proinflammatory macrophages transition into a phenotype that drives tissue repair through the production of effectors such as growth factors. This orchestrated sequence of regenerative inflammatory events, which we termed Regeneration-Promoting Program (RPP), is essential for proper repair. However, it is not well understood how specialized repair-macrophage identity develops in the RPP at the transcriptional level and how induced macrophage-derived factors coordinate tissue repair. Gene expression kinetics-based clustering of blood circulating Ly6Chigh, infiltrating inflammatory Ly6Chigh, and reparative Ly6Clow macrophages, isolated from injured muscle, identified the TGF-beta superfamily member, GDF-15, as a component of the RPP. Myeloid GDF-15 is required for proper muscle regeneration following acute sterile injury, as revealed by gain- and loss-of-function studies. Mechanistically, GDF-15 acts both on proliferating myoblasts and muscle-infiltrating myeloid cells. Epigenomic analyses of upstream regulators of Gdf15 expression identified that it is under the control of nuclear receptors RXR/PPARg. Finally, immune single-cell RNA-seq profiling revealed that Gdf15 is coexpressed with other known muscle regeneration-associated growth factors, and their expression is limited to a unique subpopulation of repair-type macrophages (Growth Factor-Expressing Macrophages, GFEM).

ORGANISM(S): Mus musculus

PROVIDER: GSE164722 | GEO | 2021/10/27

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2021-11-16 | GSE182455 | GEO
2021-11-03 | GSE161467 | GEO
2014-08-01 | E-GEOD-59948 | biostudies-arrayexpress
2019-02-01 | GSE114291 | GEO
2015-09-29 | GSE73473 | GEO
2024-12-01 | GSE274966 | GEO
2014-08-01 | GSE59948 | GEO
2024-04-24 | GSE246235 | GEO
2024-04-24 | GSE246152 | GEO
2017-02-19 | GSE55252 | GEO